人参皂甙Rb1对体外培养雪旺细胞作用的实验研究

目的研究人参皂甙Rb1对体外培养雪旺细胞增殖分化的影响,探讨其促进神经再生的作用和机制。方法取8个月月龄的新西兰兔的坐骨神经雪旺细胞体外培养第2代,加入不同浓度的人参皂甙Rb1,继续培养10d,显微镜观察计数,绘制各自的增殖曲线。在36h和72h对各组细胞进行流式细胞分析。结果活细胞计数显示:含20μg/ml人参皂甙Rb1组的倍增时间为5.3d,明显优于对照组(P<0.01)。用含20μg/ml人参皂甙Rb1培养36h、72h后雪旺细胞增殖的流式细胞分析,处于S期的雪旺细胞较对照组明显增高(P<0.01)。结论人参皂甙Rb1具有促进体外培养雪旺细胞快速增殖分化的作用,有助于损伤神经的再生。

Experimental study of the effects of ginsenoside Rb1 on the cultured Schwann cells in vitro

Objective To study the effects of Ginsenoside Rb1 on the proliferation and differentiation of Schwann cells, and examine the mechanism of nerve regeneration induced by GinsenosideRb1.Methods The Schwann cells, derived from the sciatic nerve of New Zealand rabbits of 8 months age, was cultured in vitro and the second generation was used. Ginsenoside Rb1 of different concentration was added into the culture medium for 10 days. Number of cells was counted by contrast microscope and proliferation curves were drawn individually. Furthermore, analysis of cells at 36 h and 72 h were made by flow cytometer.Results Double proliferation time of Schwann cells cultured in 20ug/ml Ginsenoside Rb1 was 5.3 days and had obvious difference from the control group (P<0.01).The number of Schwann cells at S period was increased significantly when cultured with 20ug/ml Ginsenoside Rb1 in 36 h and 72 h (P<0.01).Conclusion Ginsenoside Rb1 has the function of stimulating proliferation and differentiation of Schwann cell in vitro, suggesting the important role of the Ginsenoside Rb1 in nerve regeneration.