| 人源抗HIV—1整合酶单链抗体基因的构建及表达 |
| |
| 引用本文： | 胡宝成 Duan.,LX. 人源抗HIV—1整合酶单链抗体基因的构建及表达[J]. 军事医学科学院院刊, 1998, 22(4): 241-247 |
| |
| 作者姓名： | 胡宝成 Duan. LX |
| |
| 作者单位： | 军事医学科学院生物工程研究所 |
| |
| 摘 要： | 获得人源抗ＨＩＶ－１整合酶单链抗体基因并在大肠杆菌及细胞中进行表达。方法：从人的抗ＨＩＶ－１整合酶的Ｆａｂ抗体基因片段中通过ＰＣＲ扩出此抗体的重链可变区和轻链可变区基因，经一弹性连接肽连接构建成人源抗ＨＩＶ－１整合酶的单链抗体基因。
|
| 关 键 词： | 整合酶 单链抗体 艾滋病毒 基因治疗 |
Construction and expression of human anti HIV 1 integrase single chain antibody gene |
| |
| Hu Baocheng,Duan Lingxun ,Roger J Pomerantz Institute of Biotechnology,Center of Molecular Genetics,Beijing Center for Human Virology,Jefferson Medical College,Thomas Jefferson University,Philadelphia,PA. Construction and expression of human anti HIV 1 integrase single chain antibody gene[J]. Bulletin of the Academy of Military Medical Sciences, 1998, 22(4): 241-247 |
| |
| Authors: | Hu Baocheng Duan Lingxun Roger J Pomerantz Institute of Biotechnology Center of Molecular Genetics Beijing Center for Human Virology Jefferson Medical College Thomas Jefferson University Philadelphia PA |
| |
| Affiliation: | Hu Baocheng,Duan Lingxun *,Roger J Pomerantz * Institute of Biotechnology,Center of Molecular Genetics,Beijing 100071 * Center for Human Virology,Jefferson Medical College,Thomas Jefferson University,Philadelphia,PA19107 |
| |
| Abstract: | |
| |
| Keywords: | integrase HIV 1 sFv expression gene therapy |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
