人绒毛膜癌裸鼠原位移植模型的建立

目的建立人绒毛膜癌裸鼠原位移植瘤模型。方法培养人绒毛膜癌细胞系JAR,制备JAR单细胞悬液,给5只8周龄BALB/c裸鼠经皮下注射建立皮下移植瘤模型。待裸鼠皮下成瘤后,无菌条件下取瘤组织并切成1 mm^3组织块,通过手术方式植入10只10周龄BALB/c裸鼠子宫腔内,裸鼠濒死状时4%水合氯醛(10 g/kg)腹腔注射麻醉处死,观察子宫成瘤及腹腔转移情况。解剖取子宫原位移植瘤、腹腔内转移瘤、腹腔淋巴结及其他脏器组织标本,通过组织病理学检查进行鉴定。结果10只BALB/c裸鼠中共有7只裸鼠子宫内可见移植瘤肿块形成,其中2只可同时观察到子宫移植瘤和腹膜转移瘤。在病理学形态和结构上,皮下移植瘤模型、原位移植模型和腹膜转移瘤的瘤细胞与人绒毛膜癌细胞系JAR一致。结论成功建立人绒毛膜癌JAR细胞的BALB/c裸鼠原位移植瘤模型。

Establishment of orthotopic transplantation model of human choriocarcinoma in nude mice#br#

Objective Establishment of orthotopic transplantation tumor model of human choriocarcinoma in nude mice.Methods Human choriocarcinoma cell line JAR was cultured and the single cell suspension was subcutaneously injected into five 8-week-old BALB/c nude mice to establish subcutaneous xenograft model. After subcutaneous tumor was formed in nude mice, the tumor tissue was taken under aseptic conditions and cut into 1 mm³ tissue blocks, and then implanted into the uterine cavity of ten 10-week-old BALB/c nude mice by surgery. The nude mice were anesthetized and killed by anesthesia, and the uterine tumor formation and abdominal metastasis were observed. Anatomical uterine orthotopic transplantation tumors, intra-abdominal metastases, abdominal lymph nodes and other organ tissues were dissected and identified by histopathological examination.Results A total of 7 nude mice in 10 BALB/c nude mice showed a tumor mass formation in the uterus, and 2 of them could simultaneously observe uterine xenografts and peritoneal metastasis. The pathological morphology and structure of the tumor cells in the subcutaneous xenograft model, orthotopic transplantation model and peritoneal metastasis were consistent with the human choriocarcinoma cancer cell line JAR.Conclusion The BALB/c nude mice orthotopic transplantation model of human choriocarcinoma JAR cells was successfully established.