孕期手机辐射暴露后大鼠仔鼠前额叶皮质多巴胺D1亚型受体的表达变化

目的 探讨孕期手机辐射暴露对大鼠仔鼠前额叶皮质多巴胺D₁亚型受体（D₁DR）表达的影响。方法 30只妊娠大鼠随机分为3组：正常对照组、低剂量辐射组和高剂量辐射组。各组新生鼠随机分别留养80只，分别于出生后第1、10、40和60天取脑前额叶皮质待检。采用免疫组织化学方法检测D₁DR表达的位置及强度，采用Real-time PCR方法检测D₁DR mRNA 的相对表达。结果 实验结果显示,D1DR表达在神经元的胞质、胞膜和轴突，两个辐射组的表达强度均显著低于正常对照组(P<0.05），且高剂量辐射组显著低于低剂量辐射组（P<0.05）；Real-time PCR结果与免疫组织化学方法实验结果一致。 结论 孕期手机辐射暴露可导致仔鼠前额叶皮质的D₁DR表达水平降低。

Changes on dopamin D1 receptor expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy#br#

Objective To observe the changes of dopamin D₁ receptor (D₁DR) expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy.Methods Thirty pregnant rats were randomly divided into three groups namely normal control group, low-dose radiation group and high-dose radiation group.Eighty newborn rats were randomly kept in each group. The offspring rats’prefrontal cortices were collected at day 1, day 10, day 40 and day 60 after birth. The position and intensity of D₁DR were detected by immunohistochemistry, and the relative expression of D₁DR mRNA was detected by Real-time PCR.Results The experimental result showed that D₁DR was expressed in the cytoplasm, membrane and axon of neurons. The expression intensity of D₁DR of the two radiation groups was significantly lower than that of the normal control group(P<0.05) and the expression intensity on D₁DR of the high-dose radiation group was significantly lower than that of the low-dose radiation group(P<0.05). The result of Real-time PCR was consistent with those of immunohistochemistry.Conclusion Exposure to mobile phone radiation during pregnancy could result decreased D1DR expression level in the prefrontal cortex of offspring rats.