β-纤维蛋白原基因启动子区Hae Ⅲ多态性与缺血性脑血管病患者血浆纤维蛋白原浓度关系的研究

目的 研究中国汉族人群中β-纤维蛋白原基因启动子区HaeⅢ-455G／A多态性与血浆纤维蛋白原浓度和缺血性脑血管病的关系。方法 随机选取134名缺血性脑血管病患和166名对照人群，用PCR(聚合酶链式反应)和RFLP(限制性片段长度多态性)方法，分析受检的基因型，用Follin酚法测定血浆纤维蛋白原的浓度。结果 对照组中AA纯合子的血浆纤维蛋白原浓度(34lmg/dL)高于GA杂合子(298mg/dL)和GG纯合子(298mg／dL）的血浆纤维蛋白原浓度，缺血性脑血管病患组中AA纯合子的血浆纤维蛋白原浓度(353mg/dL)高于GA杂合子(287mg/dL)和GG纯合子(302mg/dL)的血浆纤维蛋白原浓度。然而，β-纤维蛋白原基因启动子区HaeⅢ-455G／A多态性与缺血性脑血管病无相关性。结论 β-纤维蛋白原基因启动子区HaeⅢ-455G／A多态性虽可影响血浆纤维蛋白原浓度，但并非是缺血性脑血管病的一个重要遗传决定因子。

Relationship between β-fibrinogen promoter -455G/A (HaeⅢ) polymorphism and plasma fibrinogen of the patients with ischemic cerebrovascular disease

Objective The -455G/A (HaeⅢ) polymorphism of the β-fibrinogen gene influences levels of plasma fibrinogen.We determined whether it influences risk ofischemic cerebrovascular disease. Methods We accumulated 134 acute ischemiccerebrovascular disease (ICVD) cases and compared their -455G/A status with that in a control group (n=166). Furthermore,we analysed the β-fibrinogen gene -455A/G polymorphism for all subjects by PCR-RFLP with the restrictive enzyme HaeⅢ.Results Plasma fibrinogen was higher in AA homozygous participants (341 mg/dL) than in persons carrying the G allele: GA(290 mg/dL), GG (298 mg/dL) in the control group. Plasma fibrinogen was also higher in AA homozygous patients (353mg/dL) than in cases carrying the G allele: GA (287 mg/dL), GG (302 mg/dL) in the ICVD group. However, -455G/Apolymorphism of the β-fibrinogen gene was found not to be significantly associated with ICVD. Conclusion Although aminor effect on plasma fibrinogen cannot be excluded, -455G/A polymorphism of the β-fibrinogen gene does not appear tobe an important genetic determinant of ICVD.