荧光原位杂交法在膀胱尿路上皮癌诊断中的应用

目的：探讨荧光原位杂交法（FISH）在膀胱尿路上皮癌诊断中的应用。方法：选取20例非尿路上皮癌和40例膀胱尿路上皮癌的人群尿液作常规尿脱落细胞学检查和FISH检测。结果：FISH技术的敏感性为82．5％，显著高于常规尿脱落细胞学的敏感性25．0％（P〈0．05）；FISH技术和常规脱落尿细胞学检查的特异性均为100％，两者在特异性方面差异无统计学意义（P〉0．05）。结论：荧光原位杂交法在膀胱尿路上皮癌诊断中的特异性与常规尿脱落细胞学检查一致，但其敏感性显著高于常规尿脱落细胞学检查，所以，FISH技术更有望成为膀胱尿路上皮癌无创性的诊断和检测手段。

The Application of Fluorescence in Situ Hybridization in the Diagnosis of Urothelial Carcinoma Of Bladder

Objective：To discuss the application of fluorescence in situ hybridization （FISH） in the diagnosis of urothelial carcinoma of bladder. Methods：60 volunteers were divided into two groups： 40 patients with urothelial carcinoma of bladder and 20 healthy controls. Those urine were analyzed by means of cytology and FISH. Results： Overall sensitivity of FISH was significantly higher than that of urine cytology（82.5.0 % vs. 25.0% P〈0.05）. Specificities of urine cytology and FISH were 100%, was no significant difference（P〉0.05）. Conclusions：Specificity of FISH was the same as that of urine cytology in the diagnosis of urothelial carcinoma of bladder, but sensitivity of it was significantly higher than that of urine cytology. So FISH can will be one of the non-invasive methods as a supplementary technique for the diagnosis of urothelial carcinoma of bladder.