| HGPRT缺失和抗8-AG的人肺癌细胞(A_(549))突变株的建立 |
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| 引用本文： | 肖扬名,胡昌智,施凯,罗敏华. HGPRT缺失和抗8-AG的人肺癌细胞(A_(549))突变株的建立[J]. 中南大学学报(医学版), 1997, 0(2) |
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| 作者姓名： | 肖扬名 胡昌智 施凯 罗敏华 |
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| 作者单位： | Department of Microbiology，Hunan Medical University，Changsha 410078 |
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| 摘 要： | 用ＭＮＮＧ处理Ａ５４９细胞和８－ＡＧ软琼脂集落形成方法，成功地筛选一株抗高浓度８－ＡＧ和完全缺失ＨＧＰＲＴ活性的人肺癌细胞突变株。该株细胞能抵抗８－ＡＧ的毒性作用，对ＨＡＴ选择培养基十分敏感。从而为体细胞融合提供亲本细胞。
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| 关 键 词： | 细胞.培养的；A_（549）.细胞；细胞株.转化的；次黄嘌呤；磷酸核糖转移酶；阿扎鸟嘌呤；诱变；方法 |
ESTABLISHMENT OF MUTANT STRAIN FROM HUMAN LUNG CANCER CELL[H4 CANCER CELL (A549) WITH CHARACTERISTICS OF RESISTANCE TO 8 AG AND HGPRT DEFICIENCY |
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| Xiao Yangming Hu Changzhi Shi Kai Luo Minhua. ESTABLISHMENT OF MUTANT STRAIN FROM HUMAN LUNG CANCER CELL[H4 CANCER CELL (A549) WITH CHARACTERISTICS OF RESISTANCE TO 8 AG AND HGPRT DEFICIENCY[J]. Journal of Central South University. Medical sciences, 1997, 0(2) |
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| Authors: | Xiao Yangming Hu Changzhi Shi Kai Luo Minhua |
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| Abstract: | |
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| Keywords: | Cells cultured A549 cell * cell line transformed hypoxanthine guanine phosphoribosyltransferase mutagenesis methods |
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