上海市浦东新区副溶血弧菌病原学与分子流行病学特征分析

目的 了解上海市浦东新区副溶血弧菌病原学与分子流行病学特征。 方法 运用玻片凝集法对505株副溶血弧菌菌株进行血清学分型,运用K-B纸片法对菌株进行12种抗生素药敏试验,同时对菌株基因组DNA经限制性内切酶NotⅠ酶切后进行脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分子分型,利用BioNumerics Version 6.64 分析软件对图谱进行聚类分析,初步建立PFGE分子分型数据库。 结果 505株菌株中有117株血清型未能分型,O3:K6为患者分离株中的优势血清型,其中69.14%(56/81)的食物中毒分离株和61.87%(185/299)的散发病例分离株血清型为O3:K6型;监测食品分离株血清型分布呈现多样性,无优势菌株。99.41%(502/505)菌株对氨苄西林耐药。505株菌株共获得221个不同的PFGE带型,有26株PFGE未能分型。监测食品分离株的PFGE呈现遗传多样性,无优势带型,并且与散发及食物中毒患者分离株的PFGE带型不同。食物中毒与散发病例分离株的优势带型相同。耐2种或以上抗生素的菌株与其他菌株的PFGE型不相同,相同PFGE带型内的菌株血清型相同,不同时间、不同腹泻监测点之间存在完全相同PFGE条带。 结论 浦东新区未出现多重耐药菌株,存在多起疑似聚集性病例事件,但与监测食品分离株的遗传谱系关系较远。

Etiology and molecular epidemiology of Vibrio parahaemolyticus in Pudong, Shanghai

Objective To understand the etiological and molecular epidemiological characteristics of Vibrio parahaemolyticus in Pudong new district. Methods Serotyping was conducted for 505 V. parahaemolyticus isolates by using slide serum agglutination test. The drug susceptibility of the isolates to 12 antibiotics was performed by using K-B method. PFGE molecular subtyping was done after the genome DNA digested by restriction enzyme NotI. PFGE pattern clustering analysis was conducted by using BioNumerics Version 6.64. Results The serotyping of 117 of 505 isolates failed. Serotype O3:K6 was predominant in isolates from food poisoning samples (69.14%) and samples from sporadic infection cases (61.87%). No predominant serotype was detected in food samples collected in routine surveillance. In these isolates, 502 (99.41%) were resistant to ampicillin. And in these isolates, 221 PFGE patterns were detected, the PFGE typing failed for 26 isolates. No predominant PFGE pattern was detected in food samples from routine surveillance and the PFGE patterns of these isolates differed with those of the isolates from food poisoning samples and sporadic infection samples. The isolates from food poisoning samples and sporadic infection samples shared the same predominant PFGE patterns. The PFGE patterns of the isolates which were resistant to ≥2 antibiotics differed with those of other isolates. The isolates with same PFGE pattern shared same serotype. Same PFGE patterns were detected in different time or at different surveillance sites. Conclusion No multi-drug resistant isolate of V. parahaemolyticus was detected in Pudong. There were clustering of the infection, but the isolates from these infections had far genetic distance with the isolates from routine surveillance.