多株创伤弧菌的基因分型和药敏试验

目的 了解海产品中分离的创伤弧菌的基因分子流行病学特征和药敏试验结果,为进一步研究创伤弧菌和临床用药提供依据。 方法 以聚合酶链反应(PCR)方法对海产品中分离的创伤弧菌进行核酸检测与基因型分型,用ATB细菌鉴定仪配套的药敏卡对创伤弧菌进行药敏试验。 结果 海产品中分离的创伤弧菌均为溶血素A基因(vvhA)核酸阳性,vcgC/E和16S rRNA A/B分型结果显示,共有5种基因型别,分别为CB型、CAB型、EA型、CA型和EAB型。共检测出1株BT2核酸阳性,1株SerE核酸阳性。药敏试验显示,该菌对氨基糖苷类和头孢菌素类抗生素有耐药株。 结论 海产品中的创伤弧菌基因型呈多样性,以CB型为主,EAB型和CAB型同为国内首次报道的基因型。应加强对海产品中创伤弧菌的监测和耐药分析,预防创伤弧菌感染的暴发。

Genotyping and drug susceptibility test of Vibrio vulnificus strains

Objective To understand the molecular epidemiological characteristics and drug susceptibility of Vibrio vulnificus in marine products. Methods Polymerase chain reaction (PCR) was conducted to carry out the nucleic acid detection and genotyping of V. vulnificus strains isolated from marine products, and the drug susceptibility of these isolates was tested by ATB method. Results All V. vulnificus strains were positive for hemolysis A gene (vvhA). Five genotypes were detected, they were CB type, CAB type, EA type, CA and EAB type. One BT2 nucleic acid positive strain and one SerE nucleic acid positive strain were detected. The drug susceptibility test results showed that these isolates were resistant to aminoglycosides and cephalosporins. Conclusion The genotypes of V. vulnificus isolates from marine products varied,but CB type predominated,EAB type and CAB type were reported for the first time in China. It is necessary to strengthen the detection of V. vulnificus in marine products and monitoring of its drug susceptibility to prevent the outbreak of V. vulnificus infection.