| 亚甲蓝光敏法对人血浆中病毒的灭活研究 |
| |
| 引用本文： | 张文福,袁庆霞,王宏霞,李成文,刘育京. 亚甲蓝光敏法对人血浆中病毒的灭活研究[J]. 中国消毒学杂志, 1999, 16(2): 65-68 |
| |
| 作者姓名： | 张文福 袁庆霞 王宏霞 李成文 刘育京 |
| |
| 作者单位： | 军事医学科学院微生物流行病研究所北京 100071 |
| |
| 摘 要： | 向人血浆中加入１μｍｏｌ／Ｌ亚甲蓝，以１００００ｌｘ强度的溴钨灯光照射５ｍｉｎ，可灭活１０６５ＴＣＩＤ５０的水泡性口炎病毒与１０７４１ＴＣＩＤ５０的辛德毕斯病毒；作用２ｍｉｎ可灭活１０５０９ＴＣＩＤ５０的人免疫缺陷１型病毒。但作用６０ｍｉｎ仍不能破坏ＨＢｓＡｇ抗原性。
|
| 关 键 词： | 亚甲蓝光敏法 血浆消毒 辛德毕斯病毒 水泡性口炎病毒 人免疫缺陷1型病毒 病毒灭活 HBsAg |
| 修稿时间： | 1998-06-30 |
STUDY ON INACTIVATION OF VIRUS IN HUMAN PLASMA BY METHYLENE BLUE PHOTODYNAMIC METHOD |
| |
| Zhang WenfuYuan QingxiaWang HongxiaLi ChengwenLiu Yujing. STUDY ON INACTIVATION OF VIRUS IN HUMAN PLASMA BY METHYLENE BLUE PHOTODYNAMIC METHOD[J]. Chinese Journal of Disinfection, 1999, 16(2): 65-68 |
| |
| Authors: | Zhang WenfuYuan QingxiaWang HongxiaLi ChengwenLiu Yujing |
| |
| Abstract: | Experimental observation on efficacy of combination of methylene blue and light irratiation (methylene blue photodynamic method) in inactivating the viruses in human plasma in vitro was carried out in laboratory. Adding methylene blue 1 mol/L to human plasma and irradiation with the light from bromine tungsten lamp at 10 000 lx intensity for 5 min could inactivate 1065TCID50of vesicular stomatitis virus (VSV) and 10741TCID50of Sindbis virus (SV). Exposure for 2 min could inactivate 105.09TCID50of HIV-1, while the inactivating efficacy of either methylene blue or light alone was very low. However, this combination with a 60 min exposure time had no obvious destructive effect on HBsAg antigenicity. |
| |
| Keywords: | methylene blue photodynamic methodplasma disinfectionSindbis virus vesicular stomatitis virusHIV-1virus inactivationHBsAg |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
