单核细胞趋化蛋白-4在类风湿关节炎中的表达及临床意义

目的：研究CC亚族趋化因子单核细胞趋化蛋白-4（MCP-4/CCL13）在类风湿关节炎（RA）患者外周血的表达水平,并分析MCP-4的水平与疾病活动性的关系,以探讨MCP-4在RA发病机制中的作用。方法：应用酶联免疫吸附实验（ELISA）定量方法测定40例RA患者、20例其它自身免疫性疾病患者和20例正常健康对照者血清MCP-4水平。分析RA组血清MCP-4水平是否与ESR、CRP、RF及双手放射学检查等指标具有相关性。结果：RA组血清MCP-4水平为（203.79±18.64）pg/mL,其它自身免疫性疾病对照组血清MCP-4水平为（207.76±40.37）pg/mL,正常健康对照组血清MCP-4水平为（125.13±11.08）pg/mL。RA组、其它自身免疫性疾病对照组血清MCP-4水平与正常健康对照组相比均有统计学意义（P〈0.05）,RA组与其它自身免疫性疾病对照组间无统计学意义（P=0.787）,RA活动期患者血清MCP-4水平（214.86±24.46）pg/mL和非活动期患者血清MCP-4水平（190.27±29.11）pg/mL比较无统计学意义（P=0.065）。RA组血清MCP-4水平与ESR、CRP、RF及双手X片分期无相关性。结论：RA组的血清MCP-4水平高于正常健康对照组，但与ESR、CRP、RF及双手X片分期无相关性。MCP4可能参与了RA的发病过程。

Expression of Monocyte Chemoattractant Protein-4 in Rheumatoid Arthritis and its Clinical Significance

Objective：To study the expression of C-C chemokine monocyte chemoattractant protein-4（MCP-4/CCL13） in the serum of patient with rheumatoid arthritis（RA） and its relation with the degree of the disease activity.Methods：Totally 80 specimens of serum were obtained from 40 patient with RA,20 patients with the other autoimmune diseases and 20 normal controls.MCP-4 in serum was measured using ELISA.Results：Concentration of MCP-4/CCL13 in serum from RA patients and other autoimmune diseases patients was higher than that in serum from norreal controls [（203.79±18. 64）pg/mL, vs （207.76±40.37）pg/mL, vs （125.13±11.08）pg/mL] （P〈0.05）, and no difference was found in concentrations of MCP-4/CCL13 between RA patients and other autoimmune diseases patients （P=0. 787） and between the patients in active phase and remission phase [（214.86 ± 24.46）pg/mL, vs （190.27± 29. 11）pg/mL]. Conclusion： Concentration of MCP-4/CCL13 in serum from RA patients is higher than that in serum from normal controls, MCP-4 might play an important role in the pathogenesis of RA, but no correlation with clinical disease activity index.