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贝克曼CX系列全自动生化分析仪电解质试剂盒的研制与应用
引用本文:王林川,;于燕,;马列婷,;李和平. 贝克曼CX系列全自动生化分析仪电解质试剂盒的研制与应用[J]. 华西医学, 2009, 0(10): 2646-2648
作者姓名:王林川,  于燕,  马列婷,  李和平
作者单位:西安交通大学医学院第一附属医院检验科,西安,710061;西安市红十字会医院检验科,西安,710054
基金项目:陕西省科技功关项目(2006K11-G4)
摘    要:目的:研制贝克曼CX系列全自动生化分析仪电解质配套试剂盒。方法评价自配电解质试剂盒的主要性能指标:准确度、精密度、线性范围、稳定性和相关性,并与原装试剂进行临床标本测定结果比较。结果自配试剂盒测定定值血清Na^+、K^+、Cl^-平均相对偏差均小于2%;批内精密度、批间精密度较好,变异系数(%)均小于2%;与原装试剂测定结果高度正相关,相关系数r均大于0.99。结论自配试剂完全可以取代进口试剂进行临床应用。

关 键 词:贝克曼CX生化分析仪  电解质  自配试剂

Preparation and Application of ISE Reagent Kit for Beckman CX Series Automatic Chemical Analyzer
Affiliation:WANG Lin-chuan,YU Yan,MA Lie-tin,et al.(1.Department of Clinical Laboratory,the first Affiliated Hospital of Xi,an 710061,China;2.Laboratory,the Red Cross Hospital of Xi an,710054,China )
Abstract:Objective:To develop ISE(ion selective electrode) reagent for Beckman CX series automatic chemistry analyzer.Methods:Validation the main performances of self-made reagent,including accuracy,precision,linear range,stability,and relevance,and carried clinical comparison test with the original reagent kit.Results:The main performances of self-made reagent achieved the experimental anticipated request.As analyzing the valued serum,the average relative deviations of Na^+,K^+,Cl^-were less than 2%;within-run precision and between-run precision were good,coefficient of variations were less than 2%;the self-made reagent was highly correlated with the original reagent kit,correlation coefficient was greater than 0.99.Conclusion:Self-made reagent could substitute the original reagent kit for clinical laboratory.
Keywords:Beckman CX chemistry analyzer  electrolyte  self-made reagent
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