An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis |
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| Authors: | Ugahary Luana van de Sande Wendy van Meurs Jan C van Belkum Alex |
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| Affiliation: | The Rotterdam Eye Hospital, Rotterdam, The Netherlands. |
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| Abstract: | General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms. |
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