| 新生儿肺透明膜病与先天性人巨细胞病毒感染的相 … |
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| 引用本文： | 郭津津,赵孟陶. 新生儿肺透明膜病与先天性人巨细胞病毒感染的相 …[J]. 中华实验和临床病毒学杂志, 1998, 12(4): 364-366 |
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| 作者姓名： | 郭津津 赵孟陶 |
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| 作者单位： | 中国医科大学第二临床医院病毒室，沈阳市妇婴医院 |
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| 摘 要： | 目的 探讨新生儿肺透明膜病与先天性人巨细胞病毒（ＨＣＭＶ）感染之间的关系。方法 选择ＨＣＭＶ直接早期保守区基因序列，合成两对引物，应用套式聚合酶链式反应检测了３０例新生儿肺透明膜病患儿和１０例新生儿非肺透明膜病患儿的肺组织石腊组织石腊切片中的ＨＣＭＶ ＤＮＡ。结果 ３０例新生儿肺透明膜病患儿肺组织石蜡切片标本中有１１例（ＨＣＭＶ ＤＮＡ阳性，阳性率为３６．７％，对照组肺组织ＨＣＭＶ ＤＮＡ 阴性。
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| 关 键 词： | 肺透明膜病 病毒学 巨细胞病毒 聚合酶链反应 |
The relation of neonatal hyaline membrane disease and congenital human cytomegalovirus infection |
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| Guo Jinjin,Zhao Mengtao,Liu Lanqing,et al.. The relation of neonatal hyaline membrane disease and congenital human cytomegalovirus infection[J]. Chinese journal of experimental and clinical virology, 1998, 12(4): 364-366 |
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| Authors: | Guo Jinjin Zhao Mengtao Liu Lanqing et al. |
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| Affiliation: | Second Hospital of China Medical University, Shenyang 110003. |
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| Abstract: | To study the relation of neonatal hyaline membrane disease (HMD) and congenital human cytomegalovirus (HCMV) infection, two pairs of primers were synthesized according to the sequence of HCMV conserved region of the immediate--early(IE) gene. The nested polymerase chain reaction (nested-PCR) was used for the detection of HCMV-DNA of the paraffin-embedded lung tissues from neonatal HMD and non-neonatal HMD. 11 of 30 neonatal HMD patients were positive, the positive rate was 36.7%. Specimens of non-neonatal HMD patients were all negative. The difference between the two groups was significant (chi 2 = 5.05, P < 0.025). There is close relationship between neonatal HMD and congenital HCMV infection. |
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| Keywords: | Hyaline membrane disease Human cytomegalovirus infection Polymerase chain reaction |
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