罗格列酮对内皮细胞单核细胞趋化蛋白-1和血管细胞黏附分子-1表达的影响

目的观察罗格列酮(RGZ)对高糖及C反应蛋白(CRP)诱导的人脐静脉内皮细胞(HUVECs)的单核细胞趋化蛋白-1(MCP-1)及血管细胞黏附分子-1(VCAM-1)mRNA和蛋白表达的影响。方法体外培养HUVECs,细胞传至5代,随机分为7组,正常对照组(C组),高糖组(HG组),高糖+CRP组(HGC组),CRP组,高糖+RGZ组(HGR组),高糖+CRP+RGZ组(HGCR组),CRP+RGZ组(CRPR组)。采用RGZ 5.0μmol/L干预HUVECs24 h,RT-PCR、Western blot法分别检测干预前后MCP-1、VCAM-1 mRNA和蛋白的表达水平。结果HG组、HGC组、CRP组HUVECs中MCP-1、VCAM-1的mRNA和蛋白水平较C组显著升高(P<0.01);RGZ干预后,HGR组、HGCR组、CRPR组分别较HG组、HGC组、CRP组MCP-1、VCAM-1的mRNA和蛋白水平显著降低(P<0.01)。结论RGZ通过降低HUVECs中MCP-1、VCAM-1的表达,延缓糖尿病动脉粥样硬化的进程。

Effect of rosiglitazone on the expression of monocyte chemoattractant protein-1 and vascular cell adhesion molecule-1 in human umbilical vein endothelial cells

Objective To study the effect of rosiglitazone(RGZ) on the expression of monocyte chemoattractant protein-1(MCP-1)and vascular cell adhesion molecule-1(VCAM-1) mRNA and protein in human umbilical vein endothelial cells(HUVECs)cultured under high-glucose and C-reactive protein(CRP) environment.Methods HUVECs were cultured in vitro and MTT examination showed that RGZ at 5.0 μmol/L had little effect on HUVEC activity.HUVECs at the 5th generation were divided into four groups: control group(C),high glucose group(HG),HG plus CRP group(HGC),CRP group(CRP).The cells were treated with RGZ(5.0 μmol/L) for 24 hours except the control group.mRNA expression of MCP-1 and VCAM-1 was determined by real-time PCR;the proteins of MCP-1 and VCAM-1 were determined by Western blot.Results The mRNA and protein expression levels of MCP-1 and VCAM-1 in HUVECs of HG,HGC and CRP groups were higher than that of C group(P<0.01).The mRNA and protein expression levels of MCP-1 and VCAM-1 in cells treated with RGZ were lower than before treatment(P<0.01).Conclusion RGZ plays a key role in delaying atherosclerosis by decreasing the expression of MCP-1 and VCAM-1.