PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cutaneous leishmaniasis |
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| Authors: | Volpini Angela C Passos Valéria M A Oliveira Guilherme Correa Romanha Alvaro J |
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| Institution: | Lab. de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ; Av. Augusto de Lima 1715, Barro Preto, CEP 30190-002, MG, Belo Horizonte, Brazil. |
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| Abstract: | A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis were highly concordant (kappa=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of Leishmania in clinical specimens, infected reservoirs and vectors. |
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| Keywords: | Cutaneous Leishmaniasis Diagnostic PCR RFLP Leishmania |
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