恐惧应激大鼠血ACTH、CORT、IL-2、IL-8的含量变化

目的 探索“恐惧”情绪应激对大鼠内分泌及免疫系统的影响. 方法 根据Takashi方法建立恐惧应激模型,通过放射免疫方法检测ACTH、CORT、IL-2和IL-8,并与正常组、孤独饲养组进行对照.结果 各组大鼠ACTH、CORT均有显著差异（F 值分别10.466、13.804;均为P〈0.01）.恐惧模型组ACTH、CORT水平高于正常对照组及孤独饲养组.各组大鼠IL-8比较,存在显著差异（F=3.489,P=0.049）,恐惧模型组血清IL-8水平高于正常对照组,各组大鼠IL-2未见显著差异（F=1.355, P=0.280）. 结论 恐惧可通过激活HPA轴,升高ACTH、CORT水平这一途径调控应激反应.恐惧应激可提高IL-8水平,对机体免疫功能有抑制作用.

Effect of fear stress in rats on the contents of ACTH, CORT, IL-2, IL-8

Objective To investigate the effects of fear stress on the endocrine and immune systems of rats with fear stress. Methods The fear stress model was established according to Takashis＇ method. Then, the average contents of ACTH, CORT, IL-2, IL-8 detected by radio immunoassay method were compared in fear group, breed lonely group and normal group. Results There was significantly difference for the level of ACTH and CORT among three groups （F=10.466, 13.804; P〈0.01）. Fear stress could increase the level of ACTH and CORT, compared with other two groups （P〈0.05）.There was significantly difference for the level of IL-8 among three groups （F=3.489; P=0.049） Fear stress could increase the level of IL-8, compared with normal control group （P〈0.05）. For the level of IL-2, there was no significantly difference among three groups （F=1.355, P=0.280）. Conclusion Fear could regulate and control the stress response by activating HPA axis and increasing the level of ACTH and CORT. Fear stress could suppress the immune system by increasing the level of IL-8.