| 高密度培养大肠杆菌TB1/pMAL-hOCIFm的优化条件 |
| |
| 引用本文： | 张兴群,王粱华,焦炳华,袁勤生.高密度培养大肠杆菌TB1/pMAL-hOCIFm的优化条件[J].医学教育探索,2005(1):72-75. |
| |
| 作者姓名： | 张兴群 王粱华 焦炳华 袁勤生 |
| |
| 作者单位： | [1]华东理工大学生物反应器工程国家重点实验室,上海200237／／第二军医大学基础医学部生化与分子生物学教研室,上海200433／／东华大学化学与化工学院,上海200051 [2]第二军医大学基础医学部生化与分子生物学教研室,上海200433 [3]华东理工大学生物反应器工程国家重点实验室,上海200237 |
| |
| 基金项目： | 国家自然科学基金资助项目(30000078),国家"863"计划资助项目(2001AA215441) |
| |
| 摘 要： | 利用Bioengeering 3．7L自控式发酵罐,以分批培养和补料分批培养相结合的培养技术,高密度培养重组大肠杆菌TB1／pMAL-hOCIFm,生产重组人破骨细胞形成抑制因子成熟肽(recombinant human osteoclastogenesis inhibitory factor mature peptide,rhOCIFm)。通过对碳、氮源补加方式以及溶解氧等参数的控制,使工程菌E．coliTB1／pMAL-hOCIFm的发酵菌体OD600达到57．8,rhOCIFm与MBP融合蛋白(hOCIFm-MBP)的含量达到3．2g／L。本研究为工业化生产rhOCIFm奠定了基础。
|
| 关 键 词： | 破骨细胞形成抑制因子（OCIF） 表达 高密度培养 发酵 补料-分批培养 |
High Cell-Density Culture of E.coli TB1/pMAL-hOCIFm |
| |
| ZHANG Xing-qun.High Cell-Density Culture of E.coli TB1/pMAL-hOCIFm[J].Researches in Medical Education,2005(1):72-75. |
| |
| Authors: | ZHANG Xing-qun |
| |
| Institution: | ZHANG Xing-qun~ |
| |
| Abstract: | Batch and fed-batch culture process of E.coli TB1/pMAL-hOCIFm were carried out to produce recombinant human osteoclastogenesis inhibitory factor mature peptide (rhOCIFm) in 3.7 L Bioengineering autocontrol fermentor. High cell-density and high expression were achieved by the control of carbon/nitrogen source and dissolved oxygen. The final cell density and concentration of rhOCIFm-MBP (fusion) protein were 57.8 OD_(600) and 3.2 g/L respectively. The purity of rhOCIFm-MBP fusin protein was more than 70% after treated with affinity chromatography on amylose resin. This study provides a basic work for the production of rhOCIFm in industrial scale. |
| |
| Keywords: | OCIF expression high density culture fermentation fed-batch |
|
| 点击此处可从《医学教育探索》浏览原始摘要信息 |
| 点击此处可从《医学教育探索》下载免费的PDF全文 |
