生殖支原体和解脲脲原体与非淋菌性尿道炎相关性的研究

目的:研究生殖支原体(Mg)及解脲脲原体(Uu)与男性急性非淋菌性尿道炎(NGU)的相关性。方法:采用病例对照研究,收集性病门诊NGU病人、性病门诊有非婚性行为但无尿道炎的就诊者及健康体检者三组人群的尿道拭子,用AMPLICORCTNGPCRTest进行沙眼衣原体(Ct)的检测,巢式PCR(nPCR)和培养法进行Uu、人型支原体(Mh)的检测,nPCR及扩增产物DNA测序进行Mg的检测。结果:三组人群中Mg的检出情况分别为:25例(25%)、6例(6.4%)和0(P<0.0001),其中6例NGU病人为Ct与Mg混合感染。在NGU和门诊对照组中,Mg阳性者罹患急性NGU的危险性是Mg阴性者的3.917倍,(95%CI:1.618～9.126);在Ct阴性的NGU(NCNGU)中,Mg感染使罹患急性NGU的危险性增加7.2倍(95%CI:2.9～18.0)。nPCR检出三组人群中Uu的携带者分别为:40例(40%)、42例(44.7%)和46例(46.9%)。结论:Mg感染与男性急性NGU呈强相关,Uu与NGU无相关性。

Association of Mycoplasma genitalium and Ureaplasma urealyticum with nongonococcal urethritis

Objective:To assess the association of Mycoplasma genitalium and Ureaplasma urealyticum with nongonococcal urethritis (NGU). Methods: A case-control study was undertaken to recruit 100 male patients with acute NGU presenting to our sexually transmitted disease (STD) clinic, 94 controls presenting to the same clinic, which had nonmarital sex but without the manifestation of acute NGU and 98 subjects for health examination. Chlamydia trachomatis and Neisseria gonorrhoeae were assayed using the AMPLICOR CT/NG PCR Test (Roche Diagnostic Systems). Qualitative and quantitative cultures as well as nested PCR for U. urealyticum and M. homonis were performed. Nested PCR was applied to detect M. genitalium. Results:M. genitalium in 25(25%) of 100 NGU patients and 6 (6.4%) of 94 control subjects was detected. None of the 98 subjects for health examination (P<0.0001) were detected positive. Only 6 men with NGU were co-infected with both C. trachomatis and M. genitalium. U. urealyticum was detected by PCR in the three groups with the rates of 42%, 44.7% and 46.9% respectively. In NGU patients, M. genitalium was associated with a 3.917 -fold risk of urethritis (95% confidence interval, 1.618-9.126). In non-chlamydial NGU (NCNGU), M. genitalium was associated with a 7.2 -fold risk of urethritis (95% confidence interval, 2.9-18.0). Conclusion: M. genitalium was strongly associated with acute NGU whereas the detection of U. urealyticum was not associated with acute NGU.