Graves病患者外周血CD4^+CD25^+T细胞及Foxp3mRNA表达的变化 |
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引用本文: | 查兵兵,刘军,洪晓武,查英,王芳,丁和远,陈灶萍.Graves病患者外周血CD4^+CD25^+T细胞及Foxp3mRNA表达的变化[J].临床内科杂志,2010,27(1):37-39. |
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作者姓名: | 查兵兵 刘军 洪晓武 查英 王芳 丁和远 陈灶萍 |
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作者单位: | 1. 复旦大学上海医学院上海市第五人民医院内分泌科,上海,200240 2. 复大学上海医学院免疫学系 |
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基金项目: | 复旦大学青年骨干基金资助项目 |
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摘 要: | 目的通过比较Graves病患者和正常人外周血中CD4^+和CD8^+T细胞占淋巴细胞、CD4^+CD25^+T细胞占CD4^+T细胞的比例以及相关基因Foxp3mRNA表达的变化,探讨Graves病患者免疫机制中CD4^+CD25^+T细胞亚群所起的作用。方法选择40例初次确诊Graves病的患者和40名健康自愿者,通过流式细胞仪检测外周血CD4^+CD8^+T细胞占淋巴细胞、CD4^+CD25^和CD4^+CD25^high细胞占CD4^+T细胞的比例以及CD4^+CD25^+T细胞的荧光强度。应用Real—Time PCR检测外周血单个核细胞Foxp3mRNA的表达。结果Graves病患者外周血中CD4^+T细胞比例以及CD4^+/CD8^+的比值明显升高,而CD4^+CD25^和CD4^+CD25^highT细胞占CD4^+T细胞的比例以及CD4^+CD25^+T细胞的荧光强度与正常人比较差异无统计学意义,Graves病患者外周血单个核细胞Foxp3mRNA的表达也无明显减少。结论Graves病主要通过效应性CD4^+T细胞的体液免疫反应对甲状腺组织产生影响,CD4^+CD25^+T细胞的免疫抑制作用减弱可能仅是Graves病发病机制中的一个次要环节。
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关 键 词: | Graves病 CD4^+CD25^+T细胞 Foxp3 |
The change of CD+4 CD+25 T cells and the expression of Foxp3 mRNA in peripheral blood of patients with Graves' disease |
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Institution: | ZHA Bingbing, LIU Jun, Hong Xiaowu, et al.( Department of Endocrinology, Shanghai Fifth People' s Hospital, Fudan University, Shanghai 200240, China) |
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Abstract: | Objective To investigate the role that CD4^+CD25^+T cells play in Graves' disease by comparing the proportion of CD4^+CD25^+T cells to CD4^+ T cells as well as CD4^+ and CD8^+ T cells to lymphocytes in peripheral blood between newly diagnosed patients with Graves' disease and healthy people. Methods Flow cytometry was used to detect the proportion of CD4^+ and CD8^+ T cells to lymphocytes,the ratio of CD4^+/CD8^+, the proportion of CD4^+CD25^+, C4^+CD25^highT cells to CD4^+ T ceils as well as the mean flu- orescence intensity(MFI) of CD25 expression of these cells respectively in peripheral blood of 40 newly diagnosed patients with Graves' disease. Real-time polymerase chain reaction was performed also to measure the expression of Foxp3 mRNA. Results A significant increase in the proportion of CD4^+ T cells to lymphocytes and the ratio of CD4^+/CD8^+ was detected in peripheral blood of newly diagnosed patients with Graves' disease. However no significant statistical difference was found in the proportion of CD4^+CD25^+T, CD4^+ CD25^high T cells to CD4^+ T cells, MFI of CD25 expression as well as the expression of Foxp3 mRNA between the patients and healthy people. Conclusions Graves' disease was mainly mediated by CD4^+ T ceils. CD4^+CD25^+T cells may not take an important role in the pathogenesis of Graves' disease. |
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Keywords: | Foxp3 |
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