用石蜡切片行C3d、C4d免疫组化染色辅助诊断大疱性类天疱疮的意义

目的 探讨C3d、C4d免疫组化染色在石蜡包埋组织切片中辅助诊断大疱性类天疱疮的价值.方法 通过免疫组织化学SP法检测20例大疱性类天疱疮患者石蜡包埋组织切片中C3d、C4d的表达,并与家族性良性天疱疮、大疱性表皮松解症患者及正常皮肤进行对照.结果 20例大疱性类天疱疮患者石蜡包埋切片C3d、C4d真表皮交界基底膜处沉积率为95％(19/20),9例大疱性表皮松解症患者中真表皮交界基底膜处C3d、C4d阳性率0％(0/9),4例家族性慢性良性天疱疮患者基底膜带均为阴性.结论 石蜡包埋组织切片中,C3d、C4d免疫组化染色可以作为辅助诊断大疱性类天疱疮的方法之一.

Diagnostic value of immunohistochemical staining for C3d and C4d in paraffin sections in bullous pemphigoid

Objective To estimate the value of immunohistochemical staining for C3d and C4d in paraffin-embedded tissue sections for the auxiliary diagnosis of bullous pemphigoid (BP).Methods Paraffinembedded tissue sections from 20 patients with BP served as the experiment group, and those from lesions of 9 patients with familial benign pemphigus and 4 patients with epidermolysis bullosa as well as from normal skin served as the controls.Immunohistochemical SP method was used to measure the expressions of C3d and C4d in these paraffin sections.Results The deposition of C3d and C4d was observed in the basement membrane at the dermo-epidermal junction in 95％ (19/20) of BP tissue sections, but undetected in any of the familial benign pemphigus or epidermolysis bullosa tissue sections.Conclusion Immunohistochemical staining for C3d and C4d in paraffin-embedded tissue sections may serve as an auxiliary method for the diagnosis of BP.