CIK细胞对胃癌OCUM-2MD3细胞体内外杀瘤活性的实验研究

目的：研究正常人细胞因子激活的杀伤细胞（Cytokine-induced killer,CIK)对人胃癌细胞株OCUM－2MD3的体外细胞毒活性以及对胃癌腹膜移植模型的体内抗肿瘤作用。方法：取正常人外周血单个核细胞（Peripheral blood mononuclear cell,PBMC)，加入γ－IFN、IL－2、抗－CD3单抗和IL－1，体外诱导成CIK细胞，用流式细胞仪对细胞作动态表型分析，用MTT法测定CIK细胞体外对OCUM－2MD3的细胞毒活性，并与CD3AK作对比。在Balb/c裸小鼠腹腔内接种OCUM－2MD3细胞，观察CIK细胞对荷瘤鼠的抑瘤作用。结果：CIK细胞在培养2周左右获得大量增殖，CD3^ CD56^ 双阳性细胞大量增殖达1000倍以上；体外实验证明，CIK细胞对OCUM－2MD3有明显的细胞毒活性，对比CD3AK其抑瘤率与85％与62．8％（P＜0．05）。体内实验表明，CIK细胞能够显著抑制癌性腹水的生长，其抑瘤率可达100％；肿瘤标志物CEA含量明显降低。结论：CIK细胞是一种新型、高效的免疫活性细胞，具有较强的体内外抗胃癌细胞活性，具有明显的抑制癌性腹水的生长的作用，有可能用于临床上晚期胃癌的过继性免疫治疗。

Proliferation profile in vitro and anti-tumor effects of CIK cells against gastric cancer cell lines OCUM-2MD3 in vitro and in vivo

Objective To investigate th e cytotoxicity of CIK (Cytokine induced killer) cells from healthy donor in v itro and their anti tumor effects in Balb/c nude mice bearing OCUM 2MD3 cell s strain of ascites tumor from gastric carcinoma.Methods Periph eral blood mononuclear cells(PBMC) from healthy donors were induced to be come CIK c ells by cytokine in vitro incubation. The CIK cells were analyzed on differe nt t ime points by FACS; Compared with CD3AK cells, the cytotoxicity in vitro by MTT assays and the antitumor activity of the CIK were evaluated in Balb/c nude mice bearing OCUM 2MD3 cells from carcinoma of stomach.Results Flow cytometry analysis showed that CD3 +CD56 + T cells expanded 1 000 fold after 2 weeks in CIK culture; CIK cells possessed a higher antitumor cytotoxic activit y on OCUM 2MD3 cells in vitro than CD3AK cells; In addition, CIK cells had a stronger suppressive effect on tumor growth in Balb/c nude mice bearing OCUM 2MD3 tumor compared with CD3AK cells in vivo (Median inhibitory rates 85% vs 62.8%, P <0.05).Conclusion CIK cells are highly e fficient cytolytic effector cells which have a stronger significant suppression against growth of carcinoma of stomach in vitro and in vivo . The result provides an experimental basis for CIK to clinical application as a adoptive imm unotherapy.