温肾养血方对无排卵模型大鼠卵巢质量和形态的影响

目的 探讨温肾养血方对无排卵大鼠子宫指数、卵巢指数及卵巢组织形态的影响.方法 采用丙酸睾酮皮下注射法复制无排卵大鼠模型.将模型大鼠分为模型组,西药组,温肾养血方大、中、小剂量组,分别给予生理盐水,枸橼酸氯米芬,温肾养血方大剂量(52 mg/kg)、中剂量(26 mg/kg)、小剂量(13mg/kg)灌胃.另选22日龄大鼠作为正常组.连续灌胃21 d后,测定各组大鼠体质量、子宫指数和卵巢指数,光镜下观察卵巢组织形态.结果 与正常组比较,模型组大鼠体质量无明显变化(P＞0.05),子宫指数和卵巢指数显著降低(P＜0.05).与模型组比较,各给药组大鼠体质量和子宫指数、卵巢指数无明显变化(P＞0.05).温肾养血方大、中剂量组卵巢形态近似正常组,结构明显改善,多数卵巢可见发育期的各级卵泡,可见卵丘、卵细胞及典型黄体形成.西药组和温肾养血方小剂量组卵巢形态类似模型组,卵巢体积较小,卵巢表面苍白,无斑点,色泽苍白,卵泡数目少,卵泡较小,且形状不规则,多呈囊性扩张,无黄体.结论 温肾养血方能改善卵巢结构和促进卵泡发育.

Effects of Wenshen Yangxue Decoction on Ovarian Quality and Morphology of Anovulatory Rats

Objective To study the effects of Wenshen Yangxue Decoction (WYD, for warming the kidney and nourishing the blood) on uterine index, ovarian index, and ovarian morphology of anovulatory rats. Methods Anovulatory rats were reproduced by subcutaneous injection of testosterone propionate, and divided into model group, Western medicine group, high-dose WYD group, medium-dose WYD group, and low-dose WYD group. Rats in the above groups were given normal saline solution, clomiphene citrate, 52 mg/kg WYD, 26 mg/kg WYD, and 13 mg/kg WYD, respectively, by gavage. Some other 22-day-old rats were enrolled into normal group. After 21 days of continuous gavage, the body weight, uterine index, and ovarian index in each group were measured, and ovarian morphology was observed using light microscopy. Results Rats in the model group had significantly lower uterine and ovarian indices than those in the normal group (P<0.05), while there was no significant difference in body weight between the two groups (P>0.05). There were no significant differences in body weight, uterine index, and ovarian index between the model group and each medication group (P>0.05). Ovarian morphology in the high-dose and medium-dose WYD groups were similar to that in the normal group, with substantially improved structures, follicles at different stages of development observed in most of ovaries, and observed formation of cumuluses, eggs, and typical corpus luteum. Ovarian morphology in the Western medicine group and low-dose WYD group were similar to that in the model group, with a small ovarian size, gray and spotless ovaries, few, small, and mostly cystically dilated follicles, and no corpus luteum. Conclusion WYD can improve ovarian structure and promote follicle development.