New bone formation and osteolysis by a metastatic, highly invasive canine prostate carcinoma xenograft |
| |
Authors: | LeRoy Bruce E Thudi Nanda K Nadella Murali V P Toribio Ramiro E Tannehill-Gregg Sarah H van Bokhoven Adrie Davis Deborah Corn Stephanie Rosol Thomas J |
| |
Affiliation: | Department of Pathology, University of Georgia, Athens, GA, USA. |
| |
Abstract: | BACKGROUND: Osteoblastic metastases are commonly induced by prostate cancer. A canine prostate carcinoma xenograft (Ace-1) was developed and used to evaluate neoplastic prostate cell growth, metastasis, and effects on bone formation in nude mice. METHODS: Characteristics of the Ace-1 cells were evaluated with histopathology, radiography, and bioluminescent imaging (BLI). Immunohistochemistry and quantitative RT-PCR were used to evaluate the expression of factors important in the development of osteoblastic metastases. RESULTS: The Ace-1 cells were invasive and induced bone formation and destruction. Radiographs demonstrated a mixed osteoblastic/osteolytic reaction. Lung and lymph node metastases occurred in 30% of mice. The tumor cells expressed parathyroid hormone-related protein (PTHrP-141 isoform), cathepsin K, keratins 8/18, and vimentin, but not keratins 5/14, and were androgen receptor negative. Intracardiac (IC) injections resulted in metastases in vertebrae and long bones. CONCLUSIONS: The Ace-1 xenograft is a useful model for investigating the pathogenesis of prostate cancer invasion and mixed osteoblastic/osteolytic bone metastases. |
| |
Keywords: | prostate carcinoma osteoblastic osteolytic canine parathyroid hormone‐related protein |
本文献已被 PubMed 等数据库收录! |
|