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提高平均动脉压对猪心肺复苏后脑功能及超微结构的影响
引用本文:王胜奇,李春盛. 提高平均动脉压对猪心肺复苏后脑功能及超微结构的影响[J]. 中国危重病急救医学, 2010, 22(7): 674-679,前插1. DOI: 10.3760/cma.j.issn.1003-0603.2010.11.009
作者姓名:王胜奇  李春盛
作者单位:首都医科大学宣武医院急诊科,北京,100053;首都医科大学附属北京朝阳医院急诊科;
摘    要:Objective To investigate the effects on brain function and ultrastructure changes by elevating mean arterial pressure (MAP) with norepinephrine after cardiopulmonary resuscitation (CPR) in pigs.Methods After 4 minutes of untreated ventricular fibrillation, CPR was begun in 10 piglets, followed by defibrillation.Following the restoration of spontaneous circulation (ROSC), the animals were randomly assigned to two treatment groups: the hypertension group (HT,n = 5), in which animals were given an infusion of norepinephrine to maintain the MAP to 130% above that before ventricular fibrillation, and the normal perfusion group (NP, n=5) who received an infusion of norepinephrine to maintain the MAP to that obtained right after ROSC.Sham-operation group of 2 animals were treated identically, with the exception that neither cardiac arrest was induced nor CPR was performed, to serve as control group.Variables of hemodynamics were measured at baseline and also 4 hours after ROSC.The overall performance categories (OPC) was evaluated 24 hours after ROSC.Then, animals were sacrificed and the brains were removed for histopathological examination of cerebral cortex, CA1 region of hippocampus, cerebellar cortex, and corpus striatum for the assay of histological damage score (HDS), and apoptosis of cerebral neurons were evaluated [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)]24 hours after ROSC.The ultrastructure of neurons was characterized, using transmission electron microscopy.Results Five animals in the HT group showed good OPC (OPC 1 - 2), while 3 animals in the NP group showed good OPC (OPC 1- 3).Brain tissue from different regions was normal in sham-operation group.The HDS in the cerebral cortex and CA1 region of hippocampus in the HT group was lower than that in the NP group (cerebral cortex: 1.6±0.5 vs.2.2±0.8, hippocampus: 1.8±0.8 vs.2.8±0.5, both P<0.05).The TUNEL-positive cells in the cerebella and the CA1 region of hippocampus were significantly reduced in the HT group compared with the NP group (cerebella: 21.2±3.2 vs.38.6±3.8, hippocampus: 22.7±7.6 vs.35.0 ± 6.8, both P<0.05).With transmission electron microscopy, necrotic neurons were found in the cerebral cortex, striatum and the CA1 region of hippocampus, while in cerebella only granular cells and glial cells in the CA1 region of hippocampus showed apoptosis.The damages to neurons were significantly reduced in HT group.Conclusion Hypertension induced by norepinephrine is a safe and effective method to reduce brain damages and prevent apoptosis of neurons.

关 键 词:心搏骤停   脑灌注压   心肺复苏   凋亡   组织病理   

The effects on brain function and ultrastructure changes by elevating mean arterial pressure after cardiopulmonary resuscitation in pigs
WANG Sheng-qi,LI Chun-sheng. The effects on brain function and ultrastructure changes by elevating mean arterial pressure after cardiopulmonary resuscitation in pigs[J]. Chinese critical care medicine, 2010, 22(7): 674-679,前插1. DOI: 10.3760/cma.j.issn.1003-0603.2010.11.009
Authors:WANG Sheng-qi  LI Chun-sheng
Abstract:
Keywords:Cardiac arrestCerebral perfusion pressureCardiopulmonary resuscitationApoptosisTissue pathology
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