茶多酚对人牙周膜成纤维细胞增殖的影响

目的 观察茶多酚对人牙周膜成纤维细胞增殖的影响.方法 采用组织块培养法培养原代人牙周膜成纤维细胞并传代,经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定,取5～8代细胞用于实验;按茶多酚不同浓度分1 mg/ml(B组)、0.5mg/ml(C组)、0.25mg/ml(D组)、0.125mg/ml(E组)、0.0625mg/ml(F组)组和空白对照组(A组),采用细胞计数法、MTT法检测细胞增殖情况,流式细胞术检测细胞DNA含量.结果 不同浓度茶多酚均促进人牙周膜成纤维细胞的增殖和DNA合成(P＜0.05),茶多酚作用的最适浓度为0.5 mg/ml,最佳作用时间为48 h.结论 茶多酚对人牙周膜成纤维细胞增殖具有明显的促进作用.

Effect of tea polyphenols on proliferation of human periodontal ligament fibroblasts

OBJECTIVE:To determine the effect of tea polyphenol (TP) on the proliferation of human periodontal ligament fibroblasts (HPDLFs). METHODS: HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification (with SABC method) of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs. RESULTS: Different concentrations of TP all significantly increased the proliferation and DNA synthesis of the HPDLFs (P<0.05), and TP treatment at 0.5 mg/ml for 6 h produced the optimal effect. CONCLUSION: TP has obviously effect in promoting the proliferation of HPDLFs.