| T细胞无能与凋亡关系初探 |
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| 引用本文： | 范祖森,马宝骊,王利,柏峻. T细胞无能与凋亡关系初探[J]. 中国免疫学杂志, 1999, 15(8): 342-344 |
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| 作者姓名： | 范祖森 马宝骊 王利 柏峻 |
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| 作者单位： | 1. 中国科学院上海生物化学研究所分子生物学国家重点实验室406组,上海200031
2. 上海第二医科大学上海市免疫学研究所,上海200025 |
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| 摘 要： | 目的：探讨无能Ｔ细胞与凋亡的关系。方法：利用Ｂ７－１单抗和环孢素Ａ（ＣｓＡ）联用处理ＡＰＣ即Ｔ细胞系统，在体外诱致抗原特异性Ｔ细胞无能，用ＰＩ染色法经ＦＡＣＳ分析了无能Ｔ细胞的凋亡，用ＲＴ－ＰＣＲ法检测了凋亡细胞Ｆａｓ ｍＲＮＡ的表达水平。结果：无能Ｔ细胞在１、３、５、１０ｄ细胞凋亡的百分率依次为２．１６％１１．２８％、１９．２７％、４１．２２％。当加入１００Ｕ／ｍｌ ＩＬ－２维培养，无能Ｔ细胞为
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| 关 键 词： | T细胞无能 凋亡 FasmRNA 免疫学 |
Preliminary study on the correlation between T cell anergy and apoptosis |
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| FAN Zu Sen,MA Ba Li,WANG Li et al. Preliminary study on the correlation between T cell anergy and apoptosis[J]. Chinese Journal of Immunology, 1999, 15(8): 342-344 |
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| Authors: | FAN Zu Sen MA Ba Li WANG Li et al |
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| Affiliation: | FAN Zu Sen,MA Ba Li,WANG Li et al. Shanghai Second Medical University,Shanghai Institute of Immunology,Shanghai 200025 |
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| Abstract: | Objective:Study the correlation between T cell anergy and apoptosis.Methods:Antigen specific T cell anergy was induced by the combination B7 1mAb and cyclosporinA(CsA) in vitro.Anergic T cell apoptosis was analyzed by FACScan with PI staining. Fas mRNA expression in apoptic cells was detected with RT PCR. Results:Apoptic percentage of anergic T cells were 2.16%,11.28%,19.27%, 41.22 %during 1 d,3 d,5 d,10 d.Evident apoptosis was not observed in the anergic T cells cocultured with 100 U/ml IL 2. PHA activated anergic T cells reactivated by PHA,PMA A23187 and CD3mAb showed significant apoptosis at 24 h, their apoptic percentages were 34.64%, 21.27% ,15.07%,respectively.Fas mRNA dramatically up regulated expression in T cells and anergic T cells activated with the above stimuli. Conclusion:Anergic T cell was gradually apoptic in vitro Fas/Fasl interaction participated in the apoptosis. |
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| Keywords: | T cell anergy Apoptosis Fas mRNA |
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