怀牛膝药材的HPLC指纹图谱研究

目的:建立怀牛膝药材的高效液相色谱(HPLC)指纹图谱。方法:色谱柱为SHIMADZU shim-pack VP-ODS(250mm×4.6mm,5μm),柱温为30℃,流动相为甲醇-0.1%醋酸水溶液(梯度洗脱),流速为1.0mL·min-1,检测波长为279nm。结果:建立了怀牛膝原药材和经硫磺熏制过的怀牛膝药材的指纹图谱。12批怀牛膝原药材有24个共有峰,其中9批药材的相似度都在0.9以上,另外3批的相似度较低;11批经硫磺熏制过的怀牛膝药材有20个共有峰,其中7批药材的相似度都在0.8以上,相似度较原药材低。结论:该方法简便、准确、专属性强,可用于怀牛膝药材的质量控制。

Studies on HPLC Fingerprints ofAchyranthes bientata

OBJECTIVE： To establish an HPLC fingerprint of Achyranthes bientata. METHODS： SHIMADZU shim-pack VP-ODS C18 column（250 mm×4.6 mm, 5μm）was used with mobile phase consisted of acetonitrile-0.1% acetic acid （gradient elution） at flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃ and the detection wavelength was set at 279 nm. RESULTS： HPLC fingerprints of A. bientata and sulphur-fumigated A. bientata were established. HPLC fingerprints of A. bientata showing 24 characteristic peaks were established from 12 batches ofA. bientata. The similarity of 9 batches was above 0.9 and the other 3 batches had low similarity. The HPLC fingerprints of 11 batches of A. bientata fumigated by sulphur showed 20 common characteristic peaks. The similarity of 7 batches of A. bientata fumigated by sulphur was above 0.8 and the similarity was lower than the original medicinal sample. CONCLUSION： The method is simple, accurate and specific, and it can be used for quality control of A. bientata.