| LC-MS/MS法测定人血浆中石杉碱甲浓度的不确定度分析 |
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| 引用本文： | 张忠华,陈玟,许美娟,张军.LC-MS/MS法测定人血浆中石杉碱甲浓度的不确定度分析[J].中国药房,2011(38):3582-3585. |
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| 作者姓名： | 张忠华 陈玟 许美娟 张军 |
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| 作者单位： | 南京中医药大学附属医院临床药理科,南京市210029 |
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| 摘 要： | 目的:探讨高效液相色谱串联质谱电喷雾(LC-MS/MS)法测定人血浆中石杉碱甲浓度的不确定度评定方法。方法:用LC-MS/MS法测定人血浆中石杉碱甲浓度,通过对测试方法流程进行分析,确定不确定度来源,计算合成不确定度和扩展不确定度。结果:血浆中石杉碱甲浓度测定的扩展不确定度为9.16%。结论:本评定方法适用于LC-MS/MS法测定人血浆中药物浓度的不确定度评定测量,不确定度的评定方法确立对于血药浓度的测定具有重要意义。
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| 关 键 词： | 石杉碱甲 高效液相色谱串联质谱电喷雾法 不确定度 |
Analysis of Measurement Uncertainty of Huperzine A Content in Human Plasma by LC-MS/MS |
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| ZHANG Zhong-hua,CHEN Min,XU Mei-juan,ZHANG Jun.Analysis of Measurement Uncertainty of Huperzine A Content in Human Plasma by LC-MS/MS[J].China Pharmacy,2011(38):3582-3585. |
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| Authors: | ZHANG Zhong-hua CHEN Min XU Mei-juan ZHANG Jun |
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| Institution: | (Dept.of Clinic Pharmacology,The Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China) |
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| Abstract: | OBJECTIVE：To develop a method to evaluate the uncertainty of the determination of huperzine A in human plasma by LC-MS/MS.METHODS：The content of huperzine A in human plasma was determined by LC-MS/MS.Uncertainty sources were obtained from analysis of the whole procedure of LC-MS/MS method,and then,the combined standard uncertainty and expanded standard uncertainty were calculated accordingly.RESULTS：The expanded standard uncertainty of the determination of huperzine A content in human plasma was 9.16%.CONCLUSION：The method is suitable for uncertainty evaluation of the detection of huperzine A content in human plasma by LC-MS/MS.The establishment of the method for the evaluation of measurement uncertainty is important to the detection of plasma concentration. |
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| Keywords: | Huperzine A LC-MS/MS Uncertainty |
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