火焰原子吸收光谱法测定附子中微量元素的含量

目的:建立以空气-乙炔火焰原子吸收光谱法测定附子中铜(Cu)、铁(Fe)、锰(Mn)、铅(Pb)、镉(Cd)、铬(Cr)、锌(Zn)、镍(Ni)、钙(Ca)、镁(Mg)10种微量元素含量的方法。方法:样品经硝酸-高氯酸(4∶1)浸泡,置电热板上140℃消解处理。结果:测得各线性范围(μg·mL-1):Cu为0～3.0,Fe为0～4.0,Mn为0～1.0,Pb为0～5.0,Cd为0～0.25,Cr为0～4.0,Zn为0～2.0,Ni为0～5.0,Ca为0～4.0,Mg为0～4.0;相关系数均>0.9926;回收率在100.11%～102.62%之间;方法精密度的RSD在0.16%～2.2%之间。结论:该方法适合中药附子的Cu、Fe、Mn、Pb、Cd、Cr、Zn、Ni、Ca、Mg的含量测定,可为进一步研究附子中微量元素与其临床疗效关系提供有益依据。

Content Determination of Trace Elements in Aconitum carmichaelii by Flame Atomic Absorption Spectrometry

OBJECTIVE： To establish air-acetylene flame atomic absorption spectrometry （AAS） for the content determination of 10 trace elements including Cu, Fe, Mn, Pb, Cd, Cr, Zn, Ni, Ca and Mg in Aconitum carmichaelii. METHODS： The samples were digested with nitric-perchloric acid （4：1） on electric heating plate set at 140 ℃. RESULTS： The linear range（μg.mL-1） were as follows： 0～3.0 for Cu, 0～4.0 for Fe,0～1.0 for Mn,0～5.0 for Pb,0～0.25 for Cd,0～4.0 for Cr,0～2.0 for Zn,0～5.0 for Ni, 0～4.0 for Ca, 0～4.0 for Mg. All of the correlation coefficients were not less than 0.992 6. The recovery rates were between 100.11% and 102.62%, and the RSDs were between 0.16% and 2.2%. CONCLUSION： This method could be used to determine the content of Cu, Fe, Mn , Pb , Cd , Cr , Zn , Ni , Ca and Mg in A. carmichaelii. The results obtained will offer some basic data for the study of the relationship between trace elements and clinical efficacy ofA. carmichaelii.