HPLC法测定知柏地黄丸中菝葜皂苷元的含量

目的:建立测定知柏地黄丸中菝葜皂苷元含量的方法。方法:采用高效液相色谱法。色谱柱为AglientC18柱,流动相为甲醇-水(95:5),检测器为蒸发光散射检测器,流速为1.0mL·min-1,进样量为20μL,柱温为25℃。结果:菝葜皂苷元进样量在0.44～4.35μg范围内与峰面积积分值呈良好的线性关系(r=0.9997);平均回收率为98.63%、98.93%、98.93%,RSD=1.30%。结论:本法简便快捷,结果准确,重复性好,可用于知柏地黄丸的质量控制。

Content Determination of Sarsasapogenin in Zhibai Dihuang Pills by HPLC

OBJECTIVE： To establish the method for the content determination of sarsasapogenin in Zhibai dihuang pills. METHODS： HPLC method was adopted. The chromatographic separation was performed on Aglient C,8 column with mobile phase consisted of methanol-water （95：5） using evaporative light-scattering detector （ELSD） at flow rate of 1.0 mL.min^-1. The injection volume was 20 ktL and column temperature was 25 ℃. RESULTS： The linear range for sarsasapogenin was 0.44~4.35 μg（r= 0.999 7） with an average recovery of 98.63% ,98.93% ,98.93% （RSD=1.30%）. CONCLUSION： The method is simple and accurate with a good reproducibility for the quality control of Zhibai dihuang pills.