RP-HPLC法测定抗感颗粒中芍药苷和绿原酸的含量

目的:建立同时测定抗感颗粒中芍药苷和绿原酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为HypersilODS柱,流动相为甲醇-水-冰醋酸(28:72:0.5),流速为1.0mL·min-1,检测波长分别为235nm和327nm,进样量为10μL,柱温为25℃。结果:芍药苷进样量在1.628～4.884μg范围内与峰面积积分值线性关系良好(r=0.9996,n=5),平均加样回收率为99.97%,RSD=1.32%;绿原酸进样量在0.584～1.752μg范围内与峰面积积分值线性关系良好(r=0.9997,n=5),平均加样回收率为100.19%,RSD=1.37%。结论:该方法简便易行,结果准确稳定,可用于抗感颗粒的质量控制。

Content Determination of Paeoniflorin and Chlorogenic Acid in Kanggan Granules by RP-HPLC

OBJECTIVE： To establish the method for the content determination of paeoniflorin and chlorogenic acid in Kanggan granules. METHODS： RP-HPLC method was adopted. The determination was performed on Hypersil ODS column with mobile phase consisted of methanol-water-glacial acetic acid（28 ： 72：0.5）at a flow rate of 1.0 mL.min 1. The detection wavelength was set at 235 nm and 327 nm, respectively, and the column temperature was 25 ℃. The injection volume was 10 μL. RESULTS：The linear range of paeoniflorin was 1.628-4.884 μg（r=0.999 6, n=-5）with an average recovery of 99.97% （RSD= 1.32%）. Chlorogenic acid showed a good linear relationship at range of 0.584-1.752 μg （r=0.999 7, n=5）with an average recovery of 100.19% （RSD =-1.37% ）. CONCLUSION：The method is simple, convenient, accurate and sensitive, which can be used for the quality control of Kanggan granules.