三七提取液对阿尔茨海默病模型小鼠的保护作用研究

目的:研究三七提取液对阿尔茨海默病模型小鼠的保护作用。方法:以葡萄糖酸铝ig小鼠以复制阿尔茨海默病模型。实验分为5组,即正常、模型、石杉碱甲和三七总皂苷(PNS)高、低剂量组,ig给药,每天1次,每周5d,连续3月。复制模型同时ig给药。通过跳台法观察小鼠的被动回避性学习能力,测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)和乙酰胆碱转移酶(ChAT)活性。结果:与模型组比较,PNS高、低剂量组训练次数显著减少(P<0.01或P<0.05),潜伏期显著缩短(P<0.01);PNS高、低剂量组MDA含量显著减少,SOD和ChAT活性显著增强(P<0.01)。结论:PNS可通过降低MDA含量,增强SOD和ChAT活性的途径减缓老年痴呆病变状态。

Protective Effects of Panax notoginseng Extracts on Alzheime＇s disease Model Mice

OBJECTIVE： To study protective effects of Panax notoginseng extracts on the senile dementia model mice. METHODS： Senile dementia model was established by intragastrical administration of aluminum gluconate. Model mice were divided into 5 groups, i.e. normal group, model group, huperzine A group and P. notoginseng high-dose and low-dose groups. They were given relevant medicine intragastrically once a day for 5 days a week for 3 months to establish senile. dementia model. In step down test, ability of Passive elusion learning and memory of mice were observed, MDA content, SOD and acetylcholine shift enzyme （CHAT） activities were determined. RESULTS： Compared with model group, training times of P. notoginseng high-dose and low-dose groups reduced significantly （P〈0.01 or P〈0.05）, and incubation period shortened significantly （P〈0.01） ; MDA con- tent of P. notoginseng high-dose and low-dose groups significantly reduced, SOD and ChAT activities enhanced significantly （P〈 0.01 ）.CONCLUSION： P. notoginseng can reduce MDA content, strengthen SOD and ChAT acitivities to relieve senile dementia.