兔视网膜中蛋白激酶C的定位研究

目的：证实兔视网膜中有α、β和γ三种蛋白激酶C(protein kinase C，PKC)亚型的分布。方法；采用免疫组织化学技术，以抗PKC同功酶I(α)、Ⅱ(β)、Ⅲ(γ)单克隆抗体对兔视网膜进行PKC同功酶定位研究。 结果：兔视网膜中有PKC-α、PKC-β和PKC-γ的阳性免疫反应。PKC-α免疫反应主要呈现在内核层的双极细胞；PKC-β免疫反应主要呈现在神经节细胞层的神经节细胞；PKC-γ则在神经节细胞层、内网状层以及光感受器的外节呈弥漫性弱阳性染色。 结论：作为中枢神经系统的一部分，视网膜可以同时存在PKC-α、PKC-β和PKC-γ三种亚型。 （中华眼底病杂志，1996，12：242-244）

LOCALIZATION OF PROTEIN KINASE C IN RABBIT RETINA

PURPOSE:To verify existance of a-,~-,and 3'-protein kinase C(PKC)subspecies and
their localization in rabbit retina. METHODS: Using an immunohistoehemical technique with mono-
elonal antibodies against PKC isozymes- I (a),-I (13),and -~ (Y) to characterize the distribution of
PKC in rabbit retina. RESULTS:There is a positive immunostaining for a-,13-,and ~-PKC in rabbit
retina. The immunoreactivity of a-PKC was observed mainly in the bipolar cells of inner nuclear layer
and the outer segments of photorecptors. The positive immunostaining of 13-PKC could be seen in the
ganglion cells,inner plexiform layer,inner nuclear layer,and the outer segments of photoreceptors. A
diffuse and weak staining of Y-PKC is recognized in the ganglion cell layer,inner plexifrom layer,inner
nuclear layer, and the outer segments of photoreceptors. CONCLUSION:The protein kinase C sub-
speeies-a,-~,and-'Y are present in retina which is a part of the central nervous system