KAI1基因表达与大肠癌发生、发展的关系

 目的 探讨KAI1基因的表达与大肠癌(CRC)发生、发展的关系. 方法 采用RT-PCR法及免疫组织化学(EnvisionTM法)检测40例大肠癌组织、8例癌旁黏膜和8例正常黏膜的KAI1 mRNA和蛋白表达. 结果 正常黏膜KAI1 mRNA水平显著低于癌组织(P<0.05),癌旁黏膜略高于正常黏膜,但无显著差异;在大肠癌原发灶中,KAI1蛋白表达阳性25例(62.5%),弱阳性9例(22.5%),阴性6例(15%).KAI1 mRNA及蛋白水平在低分化、伴淋巴结转移肿瘤中表达显著降低(P<0.01,P<0.01;P<0.05,P<0.05). 结论 KAI1的异常表达可能参与了大肠癌的发生、发展,并对肿瘤的分化、淋巴结转移的判断有一定指导意义。

Relationship between Expression of KAI1 Gene and the Genesis and Development of Colorectal Cancer

Objective To explore the relationship between expression of KAI1 gene and the genesis and development of colorectal cancer(CRC). Methods The expression of KAI1 mRNA and protein were respectively detected using RT-PCR and high sensitive Envision TM immunohistochemical method in 40 cases of CRC ,8 cases of transitional mucosa and 8 cases of normal mucosa. Results The KAI1 mRNA of normal mucosa was significantly lower than that of tumor(P< 0.05),and there was no difference between the normal mucosa and the transitional mucosa on statistics although the KAI1 mRNA of latter was higher slightly than that of the former. The positive cases of KAI1 protein in primary CRC were 25 ( 62.5%),weak cases were 9( 22.5%), negative cases were 6(15%). KAI1 mRNA and protein expression reduced in the cases of low differentiation and the cases with lymph node metastasis(P< 0.01,P< 0.01;P< 0.05,P< 0.05).Conclusion The abnormal expression of KAI1 probably participates in genesis and development of CRC, and possesses clinical significance in evaluating the differentiation, lymph node metastasis of CRC.