Beclin1、PTEN在子宫内膜癌组织中的表达及其意义

背景与目的:子宫内膜癌的确切发病机制尚不明确。本研究旨在探讨抑癌基因Beclin1(BECN1)、PTEN在子宫内膜癌组织中的表达情况及其与临床病理参数之间的关系。方法:采用免疫组织化学PowerVision二步法测定79例子宫内膜癌、34例子宫内膜增殖症以及22例正常子宫内膜组织中BECN1、PTEN的表达并加以分析。结果:BECN1在正常子宫内膜组织、子宫内膜增殖症、子宫内膜癌组织中的阳性率分别为93.33%、58.82%、34.18%;PTEN的阳性率分别为93.33%、64.71%、32.91%。BECN1、PTEN在正常子宫内膜组织、子宫内膜增殖症、子宫内膜癌组织中的表达率逐渐下降差异有显著性(χ2=42.318,P<0.001;χ2=31.746,P<0.001)。BECN1表达程度与子宫内膜癌细胞分化、组织学类型相关,与手术病理分期和肌层浸润深度无关。PTEN蛋白表达与细胞分化程度、组织学类型以及肌层浸润深度有关,而与手术病理分期无关。BECN1与PTEN表达之间呈正相关。结论:BECN1、PTEN低表达与子宫内膜样腺癌的发生、发展相关。

Expression and clinical significance of Beclin1 and PTEN in endometrial carcinoma

BACKGROUND & OBJECTIVE: The pathogenesis of endometrial carcinoma is unclear. This study was to explore the expression of beclin1 (BECN1) and PTEN in endometrial carcinoma, and investigate their correlations to clinicopathologic features of endometrial carcinoma. METHODS: The expression of BECN1 and PTEN in 79 specimens of endometrial carcinoma, 34 specimens of endometrial hyperplasia, and 22 specimens of normal endometria were detected by PowerVision immunohistochemistry. Their correlations to clinicopathologic features of endometrial carcinoma were analyzed. RESULTS: The positive rates of BECN1 and PTEN were the highest in normal endometria, and diminished gradually in endometrial hyperplasia and endometrial carcinoma (93.33%, 58.82%, and 34.18%, Chi (2)=42.318, P<0.001, 93.33%, 64.71%, and 32.91%, Chi(2)=31.746, P<0.001). The expression of BECN1 was correlated to cell differentiation and histological type, but not to pathologic stage and myometrial invasion. The expression of PTEN was correlated to cell differentiation, histological type, and myometrial invasion, but not to pathologic stage. The expression of BECN1 was positively correlated to that of PTEN in endometrial carcinoma. CONCLUSION: The down-regulation of BECN1 and PTEN may be correlated to carcinogenesis of endometrioid adenocarcinoma.