|
|||||
|
|
| 共轭亚麻酸在体外细胞中的代谢与整合研究 | |
| 引用本文： | 孙海燕,袁高峰,李铎.共轭亚麻酸在体外细胞中的代谢与整合研究[J].营养学报,2012,34(5):454-457. |
| 作者姓名： | 孙海燕 袁高峰 李铎 |
| 作者单位： | 1. 浙江海洋学院食品与药学学院,舟山,316004 2. 浙江海洋学院食品与药学学院,舟山316004;浙江大学生物系统工程与食品科学学院食品科学与营养系,杭州310029 3. 浙江大学生物系统工程与食品科学学院食品科学与营养系,杭州,310029 |
| 基金项目： | 浙江省自然基金(No.LY12C20007);浙江海洋学院科研启动经费资助项目(No.21135010910) |
| 摘 要： | 目的以富含石榴酸(共轭亚麻酸异构体之一,含量达75.5%)的石榴籽油为材料,研究共轭亚麻酸在体外培养的肝癌细胞系HepG2中的代谢和整合状况。方法以含10μmol/L石榴酸处理HepG2 24h后进行脂肪酸分析。结果细胞中出现新的代谢产物,进一步通过GC-MS确认代谢产物为9顺,11反-共轭亚油酸(c9,t11-18:2),c9,t11-18:2,含量占总脂肪酸的2.78%,石榴酸含量占总脂肪酸的0.67%。结论石榴酸代谢产物酸能在体外细胞内整合,且整合到细胞内的石榴酸大部分进一步代谢成为c9,t11-18:2。 |
| 关 键 词： | 石榴酸 共轭亚麻酸 共轭亚油酸 代谢 体外 |
INCORPORATION AND METABOLISM OF CONJUGATED LINOLENIC ACID IN VITRO |
|
| SUN Hai-yan,YUAN Gao-feng,LI Duo.INCORPORATION AND METABOLISM OF CONJUGATED LINOLENIC ACID IN VITRO[J].Acta Nutrimenta Sinica,2012,34(5):454-457. | |
| Authors: | SUN Hai-yan YUAN Gao-feng LI Duo |
| Institution: | 1College of Food and Medicine,Zhejiang Ocean University,Zhoushan 316004,China;2 Department of Food Science and Nutrition,College of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310029,China) |
| Abstract: | Objective The incorporation and metabolism of conjugated linolenic acid(CLNA) in the HepG2 cell cultured in vitro were studied by using the pomegranate(Punica granatum) seed oil rich in punicic acid(one isomer of CLNA).Method The HepG2 cell was treated by 10 μmol/L punicic acid for 24h and the fatty acids were determined.Results The metabolite was confirmed as cis-9,trans-11 conjugated linoleic acid(c9,t11-18:2) by further identifying and analyzing by GC-MS.The proportion of c9,t11-18:2 and punicic acid in HepG2 cell were 2.78% and 0.67% after 24 h treatment of punicic acid respectively.The results showed that CLNA was incorporated into the HepG2 cell,and most CLNA was metabolized to c9,t11-18:2 in the HepG2 cell,presumably as result of metabolism by saturation reaction. |
| Keywords: | punicic acid conjugated linolenic acid conjugated linoleic acid metabolism in vitro |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |