基质金属蛋白酶9在肾细胞癌中的表达与患者临床预后的相关性研究

目的：检测基质金属蛋白酶9（MMP-9）在肾细胞癌中的表达，探讨其与患者临床预后的相关性。方法肾癌患者150例，另设癌旁组30例和健康对照组20例，利用免疫组织化学染色检测肾癌组织，癌旁组织及正常肾脏组织中MMP-9的表达。并联系相关随访记录。探讨两者之间的相关性。结果（1）MMP-9在肾癌组织中高表达，肾肿瘤旁组织及正常肾脏组织中低表达，肾癌组与肾肿瘤旁组织及正常肾脏组织分别比较，均有显著差异（P﹤0.05）。（2）MMP-9表达与肾癌病人的年龄，肿瘤大小没有相关性（P=0.412；P=0.083），与肿瘤分期、分级及淋巴结转移成正相关（ P=0.009；P=0.017；P=0.004）。伴有MMP-9高表达的肾癌患者无复发生存率明显低于MMP-9低表达的患者（ P﹤0.001）。结论 MMP-9在肾癌组织中呈持续高表达，与肾癌患者的临床病理指标，生物学行为和预后密切相关，是评估肾癌进展，不良预后的重要指标。

The expression of MMP-9 in renal cell carcinoma and their relathionship with the clinical prognosis of patients

Objective：To investigate the expression of matrix metalloproteinase-9（ MMP-9 ）in renal cell carcinoma and determine its clinical significances. Methods：The samples were divided into 3 groups：renal cell carcinoma group（150 cases）,paracarcinoma group（30 cases）and normal kidney group（20 cases）. The expression of MMP-9 in renal cell carcinoma tissues,paracarcinoma tissues and normal kidney tissues were detected by immunohistochemistry. Results：The expression of MMP-9 proteins in renal cell carcinoma tissues were significantly stronger than paracarcinoma tissues and normal kidney tissues,and the differences were significant（P﹤0. 05）. The expression of MMP-9 in renal cell carcinoma tissues was positively correlated with tumor stage,grade and lymph node metastasis（P=0. 009;P=0. 017;P=0. 004）. The expression of MMP-9 had no relation with the patients’age and tumor size（P=0. 412;P=0. 083）. The patients with high expression of MMP-9 had a significantly lower recurrence-free survival rate than patients with low expression of MMP-9（P﹤0. 001）. Conclusions：The expression of MMP-9 is closely correlated with clinical characteristics,biologi-cal behaviors and prognosis. The detection of MMP-9 can serve as an important marker for early diagnosis,development and prognosis of renal cell carcinoma.