| 杜氏利什曼原虫无鞭毛体蛋白基因真核表达重组质粒构建 |
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| 引用本文： | 李金福,张建国,陈建平,杨志伟,田玉,马莹,胡孝素. 杜氏利什曼原虫无鞭毛体蛋白基因真核表达重组质粒构建[J]. 寄生虫病与感染性疾病, 2007, 5(2): 61-63 |
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| 作者姓名： | 李金福 张建国 陈建平 杨志伟 田玉 马莹 胡孝素 |
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| 作者单位： | 1. 贵阳医学院寄生虫学教研室,贵州,贵阳,550004;四川大学华西基础医学与法医学院寄生虫学教研室
2. 四川大学华西基础医学与法医学院寄生虫学教研室 |
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| 摘 要： | 目的 构建杜氏利什曼原虫无鞭毛体蛋白（amastin）编码基因的真核表达重组质粒pcDNA3.1-amastin。方法 提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pcDNA3.1（＋）,构建真核表达重组质粒pcDNA3.1-amastin。结果 扩增出大小约550bp的无鞭毛体蛋白基因;重组质粒pcDNA3.1-amastin经鉴定正确。结论 成功构建杜氏利什曼原虫无鞭毛体蛋白基因真核表达重组质粒pcDNA3.1-amastin。
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| 关 键 词： | 杜氏利什曼原虫 无鞭毛体蛋白基因 重组质粒 |
| 文章编号： | 1672-2116（2007）02-0061-03 |
| 收稿时间： | 2007-02-25 |
| 修稿时间： | 2007-02-25 |
Construction of Eucaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani |
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| LI Jin-fu,ZHANG Jian-guo,CHEN Jian-ping,YANG Zhi-wei,TIAN Yu,MA Ying,HU Xiao-su. Construction of Eucaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani[J]. Parastoses and Infectious Diseases, 2007, 5(2): 61-63 |
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| Authors: | LI Jin-fu ZHANG Jian-guo CHEN Jian-ping YANG Zhi-wei TIAN Yu MA Ying HU Xiao-su |
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| Affiliation: | 1. Department of Parasitology, Guiyang Medical College, Guiyang 550004, Guizhou Province, China ; 2 . Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China |
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| Abstract: | Objective To construct eukaryotic expression recombinant plasmid of amastin gene of Leishmania donovani.Method Amastin gene was amplified from nuclear DNA of Leishmania donovani isolates and cloned into an eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid was named pcDNA3.1-amastin. Result Amastin gene of 550bp was acquired by PCR. The recombinant plasmid pcDNA3.1-amastin was correct by analysis.Conclusion The eukaryotic expression recombinant plasmid of amastin gene of Leishmania donovani was constructed successfully. |
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| Keywords: | Leishmania donovani amastin gene recombinant plasmid |
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