Coding region SNP analysis to enhance dog mtDNA discrimination power in forensic casework |
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| Institution: | 1. National Institute of Criminalistics and Criminology, Vilvoordsesteenweg 100, B-1120 Brussels, Belgium;2. University of Antwerp (Evolutionary Ecology Group), Groenenborgerlaan 171, B-2020 Antwerp, Belgium;3. Royal Belgian Institute of Natural Sciences (OD “Taxonomy and Phylogeny” and JEMU), Vautierstraat 29, B-1000 Brussels, Belgium;1. Geology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt;2. Geology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt;1. Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK;2. School of Chemistry, University of Leeds, Leeds, LS2 9JT, UK;3. Redbrick Molecular, The Bioincubator, 40 Leavygreave Road, Sheffield, S3 7RD, UK;1. Division of Legal Medicine, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan;2. Department of Legal Medicine, Kansai Medical University, Hirakata, Japan;3. Department of Legal Medicine and Bioethics, Nagoya University School of Medicine, Nagoya, Japan;4. Department of Forensic Medicine, Fukuoka University School of Medicine, Fukuoka, Japan;5. Department of Pathobiological Science and Technology, Faculty of Medicine, Tottori University, Yonago, Japan;6. Department of Forensic Medicine, Yamagata University School of Medicine, Yamagata, Japan;7. Department of Legal Medicine, Osaka City University Medical School, Osaka, Japan;1. KU Leuven, Department of Earth & Environmental Sciences, Celestijnenlaan 200E, B-3001 Heverlee, Belgium;2. Vrije Universiteit Brussel, Department of Chemistry, Analytical, Environmental and Geo-Chemistry, Pleinlaan 2, 1050 Brussels, Belgium;3. Ghent University, Department of Geology, Krijgslaan 281, S8, 9000 Ghent, Belgium;4. Montanuniversität Leoben, Lehrstuhl für Geologie und Lagerstättenlehre, Peter-Tunnerstraβe 5, 8700 Leoben, Austria;1. Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India;2. Research and Development Laboratory, National Dairy Development Board, c/o Indian Immunologicals Limited, Hyderabad 500032, India;3. Department of Biotechnology, Acharya Nagarjuna University, Guntur 522510, India;4. Peripheral Veterinary Hospital, Madhavaram Milk Colony, TANUVAS, Chennai 600051, India;5. Adviser, National Dairy Development Board, Anand, India |
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| Abstract: | The high population frequencies of three control region haplotypes contribute to the low discrimination power of the dog mtDNA control region. It also diminishes the evidential power of a match with one of these haplotypes in forensic casework. A mitochondrial genome study of 214 Belgian dogs suggested 26 polymorphic coding region sites that successfully resolved dogs with the three most frequent control region haplotypes.In this study, three SNP assays were developed to determine the identity of the 26 informative sites. The control region of 132 newly sampled dogs was sequenced and added to the study of 214 dogs. The assays were applied to 58 dogs of the haplotypes of interest, which confirmed their suitability for enhancing dog mtDNA discrimination power. In the Belgian population study of 346 dogs, the set of 26 sites divided the dogs into 25 clusters of mtGenome sequences with substantially lower population frequency estimates than their control region sequences. In case of a match with one of the three control region haplotypes, using these three SNP assays in conjunction with control region sequencing would augment the exclusion probability of dog mtDNA analysis from 92.9% to 97.0%. |
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| Keywords: | Forensics Dog Mitochondrial DNA Coding region SNP assays |
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