Evaluation of the RapidHIT™ 200, an automated human identification system for STR analysis of single source samples |
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| Institution: | 1. Department of Mathematical Sciences, Aalborg University, Denmark;2. Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark;1. Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia;2. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia;3. ESR, Private Bag 92021, Auckland 1142, New Zealand;4. National Institute of Standards and Technology, 100 Bureau Drive, MS 8980 and 8314, Gaithersburg, MD 20899, United States;1. Center for Human Identification, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX, 76107, United States;2. Sam Houston State University, Huntsville, TX, 77340, United States;3. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia;1. Swedish National Forensic Centre, Linköping, Sweden;2. Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden;3. Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden |
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| Abstract: | The RapidHIT™ 200 Human Identification System was evaluated to determine its suitability for STR analysis of single source buccal swabs. Overall, the RapidHIT™ 200 performed as well as our traditional capillary electrophoresis based method in producing useable profile information on a first-pass basis. General observations included 100% concordance with known profile information, consistent instrument performance after two weeks of buccal swab storage, and an absence of contamination in negative controls. When data analysis was performed by the instrument software, 95.3% of the 85 samples in the reproducibility study gave full profiles. Including the 81 full profiles, a total of 2682 alleles were correctly called by the instrument software, or 98.6% of 2720 possible alleles tested. Profile information was generated from as little as 10,000 nucleated cells, with swab collection technique being a major contributing factor to profile quality. The average peak-height-ratio for heterozygote profiles (81%) was comparable to conventional STR analysis, and while a high analytical threshold was required when offline profile analysis was performed (800 RFU), it was proportionally consistent with traditional methods. Stochastic sampling effects were evaluated, and a manageable approach to address limits of detection for homozygote profiles is provided. These results support consideration of the RapidHIT™ 200 as an acceptable alternative to conventional, laboratory based STR analysis for the testing of single source buccal samples, with review of profile information as a requirement until an expert software system is incorporated, and when proper developmental and internal validation studies have been completed. |
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| Keywords: | Forensic Rapid DNA testing Buccal swabs STR analysis |
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