SNP-microarrays can accurately identify the presence of an individual in complex forensic DNA mixtures |
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| Institution: | 1. Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;2. Forensic Biology Laboratory, Israel Police, Israel Police National HQ, Jerusalem 91906, Israel;3. Cellmark Forensics, Dallas, TX, USA;1. Maestría en Criminalística y Ciencias Forenses, Universidad Libre, Santiago de Cali, Colombia;2. Genes SAS Laboratory, Medellín, Colombia;3. Institute of Biology, University of Antioquia, Medellín, Colombia;1. Institute of Forensic and Anthropological Science, Seoul National University College of Medicine, Seoul, South Korea;2. Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, South Korea;1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China;2. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China;3. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, PR China;4. Department of Forensic Genetics, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China |
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| Abstract: | Common forensic and mass disaster scenarios present DNA evidence that comprises a mixture of several contributors. Identifying the presence of an individual in such mixtures has proven difficult. In the current study, we evaluate the practical usefulness of currently available “off-the-shelf” SNP microarrays for such purposes. We found that a set of 3000 SNPs specifically selected for this purpose can accurately identify the presence of an individual in complex DNA mixtures of various compositions. For example, individuals contributing as little as 5% to a complex DNA mixture can be robustly identified even if the starting DNA amount was as little as 5.0 ng and had undergone whole-genome amplification (WGA) prior to SNP analysis. The work presented in this study represents proof-of-principle that our previously proposed approach, can work with real “forensic-type” samples. Furthermore, in the absence of a low-density focused forensic SNP microarray, the use of standard, currently available high-density SNP microarrays can be similarly used and even increase statistical power due to the larger amount of available information. |
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| Keywords: | Microarray SNP Complex DNA Mixtures Forensic DNA |
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