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An optimized procedure for obtaining DNA from fired and unfired ammunition
Affiliation:1. DNA Analysis Center, Scientific Crime Detection Division 10, Royal Thai Police, Thailand;2. Forensic Science Program, Department of Applied Science, Faculty of Science, Prince of Songkla University, Thailand;1. Forensic Science SA, GPO Box 2790, Adelaide, South Australia 5001, Australia;2. College of Science and Engineering, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia;1. Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, USA;2. Department of Chemistry, University of North Texas, 1155 Union Circle #305070, Denton, TX 76203, USA;3. J. Craig Venter Institute, 4120 Capricorn Lane, La Jolla, CA 92037, USA;1. Forensic Science Program, The Pennsylvania State University, University Park, PA 16802, USA;2. Trace Evidence Department, Cuyahoga County Regional Forensic Science Laboratory, Cleveland, OH 44106, USA;3. Department of Psychiatry Division of Human Genetics, Yale School of Medicine & VA CT Healthcare System, New Haven, CT 06511, USA;1. Graduate Program in Forensic Science, Faculty of Allied Health Sciences, Thammasat University Rangsit Campus, Pathum Thani 12120, Thailand;2. Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University Rangsit Campus, Pathum Thani 12120, Thailand;3. Biology and DNA Sub-Division, Central Police Forensic Science Division, Office of Police Forensic Science, Bangkok 10330, Thailand
Abstract:Gun crimes are a significant problem facing law enforcement agencies. Traditional forensic examination of firearms involves comparisons of markings imparted to bullets and cartridge casings during the firing process. DNA testing of casings and cartridges may not be routinely done in crime laboratories due a variety of factors including the typically low amounts of DNA recovered. The San Diego Police Department (SDPD) Crime Laboratory conducted a study to optimize the collection and profiling of DNA from fired and unfired ammunition. The method was optimized to where interpretable DNA results were obtained for 26.1% of the total number of forensic casework evidence samples, and provided some insights into the level of secondary transfer that might be expected from this type of evidence. Briefly detailed are the results from the experimental study and the forensic casework analysis using the optimized process. Mixtures (samples having more DNA types than the loader’s known genotype detected or visible at any marker) were obtained in 39.8% of research samples and the likely source of DNA mixtures is discussed.
Keywords:Forensic science  Touch DNA  STR  Cartridges  Casings  Ammunition
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