沙利度胺对大鼠慢性移植物血管病的保护作用

目的探讨沙利度胺对大鼠慢性移植物血管病的保护作用。方法实验分为4组：同种移植对照组，供、受者均为Lewis大鼠，无特殊处理；异种移植对照组，Lewis大鼠接受Brown-Norway（BN）大鼠腹主动脉移植（BN-Lewis）；沙利度胺小剂量组（50mg·kg-1·d-1，BN-Lewis）；沙利度胺大剂量组（100mg·kg-1·d-1，BN-Lewis）。于移植后60d取移植动脉，分别进行组织病理学观察、测量内膜厚度，免疫组织化学法和蛋白质印迹法检测TNF-α及上皮细胞增殖细胞核抗原（PCNA）在移植动脉中的表达。结果同种移植对照组移植动脉形态正常；异种移植对照组移植动脉呈移植物血管病表现，血管内膜增厚；沙利度胺小剂量及大剂量组移植动脉呈内膜炎改变，内膜厚度比异种移植对照组减小（P〈0．05）。与异种移植对照组相比，沙利度胺小剂量组和大剂量组移植动脉TNF-α及PCNA蛋白表达量降低（均P〈0．05）。结论沙利度胺能降低移植动脉TNF-α及PCNA蛋白的表达，缓解移植动脉的纤维化进程以及内膜增生，对慢性排斥反应所致的移植物血管病具有抑制作用。

Thalidomide attenuates chronic allograft vasculopathy of rats

Objective To evaluate the effects of thalidomide on chronic allograft vasculopathy. Methods The abdominal aorta transplantation was performed with male Lewis or Brown-Norway rats as donors and male Lewis rats as recipients. The recipients were divided into 4 groups ： Group A （ Lewis- Lewis） with no treatment; Group B （BN-Lewis） with no treatment; Group C （BN-Lewis） that received thalidomide of 50 mg/kg per day; Group D （BN-Lewis） that received thalidomide of 100 mg/kg per day. Rats were sacrificed on day 60 after surgery. The grafted aortas were analyzed by histopathology, immunohistochemistry and Western blot analysis for TNF-α production and the expression of proliferating cell nuclear antigen （PCNA）. Results The aortic intima of Group A was eumorphism. The aortic intima of Group C and D was significantly thinner than that of Group B （ P 〈 0. 05 ）. TNF- α and PCNA expressions in Group C and D were significantly lower than those in Group B （P 〈 0. 05）. Conclusion Thalidomide may have beneficial effects on chronic allograft vasculopathy by reducing the levels of TNF-αand PCNA.