Excision products of TCR V{alpha} recombination contain in-frame rearrangements: evidence for continued V(D)J recombination in TCR+ thymocytes期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Excision products of TCR V{alpha} recombination contain in-frame rearrangements: evidence for continued V(D)J recombination in TCR+ thymocytes

Authors:	McCormack, Wayne T. Liu, Ming Postema, Christina Thompson, Craig B. Turka, Laurence A.

Affiliation:	¹ Department of Medicine, University of Michigan Ann Arbor, MI 48109, USA ² Department of Microbiology/Immunology, University of Michigan Ann Arbor, MI 48109, USA ³ Howard Hughes Medical Institute Ann Arbor, MI 48109, USA

Abstract:	Cortical thymocytes that express a TCR on their surface continueto express mRNA for the recombination activating genes RAG-1and RAG-2. As the expression of these genes appears to be necessaryand sufficient for V(D)J recombination to occur, this findingsuggests that productive TCR gene rearrangement may not by itselfterminate TCR recombination. To further study this question,we have utilized a polymerase chain reaction-based strategyto isolate and sequence excision products of secondary TCR ${alpha}$ generearrangement events. Our data shows that TCR ${alpha}$ excision productsfrom TCR⁺ thymocytes contain in-frame V–J joints and thussupport the concept that V(D)J recombinatorial activity maycontinue even in thymocytes which have undergone productiveTCR gene rearrangement.

Keywords:	allelic exclusion RAG-1 RAG-2 T cell receptor
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