| 人食管上皮细胞原代培养方法的研究 |
| |
| 引用本文： | 鲍春荣,丁芳宝,黄盛东,梅举,张宝仁.人食管上皮细胞原代培养方法的研究[J].山东医药,2005,45(28):9-10. |
| |
| 作者姓名： | 鲍春荣 丁芳宝 黄盛东 梅举 张宝仁 |
| |
| 作者单位： | 上海市长海医院,上海,200433 |
| |
| 基金项目： | 国家自然科学基金资助项目（30271293） |
| |
| 摘 要： | 目的探讨适用于组织工程食管研究的食管上皮细胞培养方法.方法对比食管上皮细胞在无血清培养基K-SFM及含血清培养基DMEM中的生长情况.结果食管上皮细胞在无血清培养基K-SFM中生长快,细胞形态好,不易被成纤维细胞污染.结论应用无血清培养基K-SFM培养食管上皮细胞,方法简便,适合推广应用.
|
| 关 键 词： | 细胞培养 食管 上皮细胞 培养基 无血清 |
| 文章编号： | 1002-266X（2005）28-0009-02 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-04-20 |
Study of the better medium for the culture of human esophageal epithelial cells |
| |
| Bao ChunRong;Ding FangBao;Huang ChengDong;Mei Ju;Zhang BaoRen.Study of the better medium for the culture of human esophageal epithelial cells[J].Shandong Medical Journal,2005,45(28):9-10. |
| |
| Authors: | Bao ChunRong;Ding FangBao;Huang ChengDong;Mei Ju;Zhang BaoRen |
| |
| Abstract: | Objective: To search for a kind of better medium for the culture of human esophageal epithelial cells.Methods: Human esophageal epithelial cells were derived from surgically resected esophagus of patients with esophageal cancer.The growth and proliferation of esophageal epithelial cells in serum-free medium K-SFM to serum-contained medium DMEM were compared.Results: In the serum-free medium K-SFM,the esophageal epithelial cells grew faster,had better growth shape and were less liable to be contaminated by fibroblasts.Conclusion: For primary cultureof human esophageal epithelial cells,the serum-free medium K-SFM is convenient and proper to be spread out. |
| |
| Keywords: | cell culture esophagus epithelial cells medium non-serum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
