基于网络药理学的枳实薤白桂枝汤治疗冠心病的分子机制研究

目的基于网络药理学方法研究枳实薤白桂枝汤治疗冠心病的作用机制。方法利用中药系统药理学数据库(TCMSP)筛选与枳实薤白桂枝汤相关的化学成分及其作用靶点;通过OMIM和NCBI数据库获取与冠心病相关的靶点,将疾病靶点与药物靶点进行交集,筛选得到疾病-药物成分共同靶蛋白。应用STRING平台构建蛋白-蛋白相互作用(PPI)网络模型;利用Cytoscape软件构建"复方-活性成分-作用靶点-疾病"相互作用网络;运用R语言进行KEGG通路和GO功能富集分析。结果经数据库分析共获得了1801个疾病靶点,42个药物作用靶点。对其中26个共同相关靶点(占枳实薤白桂枝汤靶点的62%)进行分析,发现共有119个生物学过程及58条通路被显著富集。结论枳实薤白桂枝汤治疗冠心病具有多靶点、多通路的特点。其作用机制可能与调控冠心病发展过程中细胞代谢与凋亡、脂质代谢等过程有关。

Molecular Mechanism of Zhishi Xiebai Guizhi Decoction in Treatment of Coronary Heart Disease Based on Network Pharmacology

Objective To analyze the action mechanism of Zhishi Xiebai Guizhi Decoction in the treatment of coronary heart disease based on network pharmacology.Methods TCMSP was used to select the chemical compounds and the effect targets related to the Zhishi Xiebai Guizhi Decoction;The targets related to coronary heart disease were screened through OMIM and NCBI databases.The common targets of disease-drug component were selected by intersecting disease targets with drug targets.A protein-protein interaction(PPI)network model was constructed using the STRING platform.The"Compound-Active Compounds-Targets-Disease"interaction network was constructed by Cytoscape software.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway and Gene Ontology(GO)functional enrichment analysis were performed by R language.Results A total of 1801 disease targets and 42 drug targets were screened out by network analysis.For 26 of the common targets(62%of the target of Zhishi Xiebai Guizhi Decoction)were analyzed.A total of 119 biological processes and 58 pathways were found to be significantly enriched.Conclusion Zhishi Xiebai Guizhi Decoction in the treatment of coronary heart disease has the characteristics of multiple targets and multiple pathways.Its mechanism may be related to the regulation of cell metabolism and apoptosis,lipid metabolism and other processes in the development of coronary heart disease.