色素上皮衍生因子及其基因多态性与脑微出血的关系

目的探讨色素上皮衍生因子(PEDF)及其基因多态性与脑微出血的关系。方法纳入2017-12—2019-09包头医学院第一附属医院神经内二科收治的脑微出血患者55例为病例组,同期健康体检者55例为对照组。采用PCR-RFLP法检测PEDF基因启动子区rs12948385的多态性,采用酶联免疫吸附法测定PEDF水平。结果2组血清色素上皮衍生因子浓度分别为112.08±36.67、152.77±41.08,差异有统计学意义(P<0.05)。多因素分析显示,PEDF是脑微出血发生的独立危险因素。色素上皮衍生因子基因启动子区rs12948385的基因型(GG、GA和AA型)与等位基因(G/A)在脑微出血组与健康对照组间的分布差异有统计学意义(P<0.05)。GA+AA型基因发生脑微出血的风险是GG型基因的6.271倍,差异有统计学意义(P<0.05)。结论脑微出血患者PEDF水平较正常人低,PEDF水平与脑微出血发生相关。PEDF基因启动子区rs12948385多态性与脑微出血相关,携带A等位基因可能增加脑微出血的发生率。

The relationship between pigment epithelial derived factors and gene polymorphism and cerebral microhemorrhage

Objective To investigate the the relationship between pigment epithelial derived factors and gene polymorphism and cerebral microhemorrhage.Methods From Dec.2017 to Sep.2019,collected 55 cases with cerebral microbleeds in the second department of neurology,the first affiliated hospital of Baotou medical college as the case group and 55 cases health checker as the control group.The PEDF gene rs12948385 G→A polymorphism was measured based on polymerase chain reaction fragment length polymorphism(PCR-RFLP).The PEDF level was determined by elisa.Results The Level of serum PEDF concentrations of the two groups were 112.08±36.67 and 152.77±41.08,respectively,showing a statistical difference(P<0.05)further multivariate and unconditional Logistic analysis showed that PEDF was an independent risk factor for the occurrence of cerebral microbleeds.The genotypes(GG,GA and AA)and alleles(G/A)of rs12948385 in the promoter region of pigment epithelial derived factor gene were significantly different between the cerebral microbleeds group and the healthy control group(P<0.05).GA+AA gene had a 5.889 times higher risk of cerebral microbleeds than GG gene(P<0.05).Conclusion PEDF level of patients with cerebral microbleeds is lower than that of normal people,and PEDF level is related to cerebral microbleeds.Polymorphism of PEDF gene promoter rs12948385 is related to cerebral microbleeds,and carrying A allele may increase the incidence of cerebral microbleeds.