不同产地葶苈子脂溶性成分的GC-MS分析

目的 对不同产地葶苈子的脂溶性成分进行气相色谱-质谱联用技术(GC-MS)分析,比较各产地葶苈子之间脂溶性成分组成和含量的差异.方法 采用加热回流提取法提取不同产地葶苈子的脂溶性成分,利用GC-MS对脂溶性成分中的化合物组成进行鉴定,面积归一化法计算各化合物的相对百分含量.结果 从三个不同产地的葶苈子脂溶性成分中共鉴定...

Analysis of the fat-soluble components of Lepidii Semen in different habitats by GC-MS

Objective To analyze the fat-soluble components of Lepidii Semen in different habitats by gas chromatography-mass spectrometry(GC-MS),and compare the composition and content of fat-soluble components among different habitats.Methods The fat-soluble components of Lepidii Semen from different habitats were extracted by heating reflux extraction method.The fat-soluble components were identified by GC-MS.The relative percentage content of each compound was calculated by area normalization method.Results Seventy compounds were identified in the fat-soluble components of Lepidii Semen from three different habitats.34,32 and 43 kinds of the fat-soluble components were identified in Lepidii Semen from Anhui,Hebei and Yanbian area of Jilin,respectively.Linolenic acid,11-eicosanoic acid,myristitc acid,palmitic acid and stearic acid were the main components in Lepidii Semen from the three habitats.The contents of these fatty acids were slightly different in the three habitats.Among them,the content of linolenic acid was the highest.The content of linolenic acidin Lepidii Semen from Yanbian was 48.67%and had the highest relative content among three habitats.Conclusion Different habitats have influence on the composition and content of fat-soluble components of Lepidii Semen.This study provides experimental basis for the quality evaluation of Lepidii Semen.