不同产地白术药材质量评价研究

目的:对浙江、湖南、安徽3个产地共9批白术药材进行质量对比研究、指纹图谱对比分析,为不同产地白术鉴别及质量评价提供参考。方法:按照《中华人民共和国药典》的要求对白术药材进行质量检验,比较3个不同产地白术药材质量差异;采用反相高效液相色谱法,测定白术指纹图谱,进行相似度分析及主成分分析。结果:3个产地的白术药材均符合标准要求;对9批白术药材的指纹图谱进行分析,选择稳定性好、吸收强、特征明显的色谱峰作为共有峰,结果共标定了7个共有指纹峰,9批样品之间的共有峰相似度> 0. 96。结论:浙江、湖南、安徽3个产地的白术药材均符合质量标准要求,指纹图谱也没有差异,证明3个产地的白术药材没有明显质量差异,无法鉴别区分。

Quality assessment of Atractylodes macrocephala from different producing areas

Objective: To compare the quality and fingerprint of nine batches of Atractylodes macrocephala from three producing areas( Zhejiang,Hunan,and Anhui),and to provide a reference for the identification and quality assessment of Atractylodes macrocephala from different producing areas. Methods: Quality inspection was performed for Atractylodes macrocephala according to the requirements in Pharmacopoeia of The People’s Republic of China. Reversed-phase high-performance liquid chromatography was performed to obtain the fingerprint of Atractylodes macrocephala,and a similarity analysis and a principal component analysis were performed. Results: Atractylodes macrocephala from the three producing areas all met the quality standard. The fingerprints of the nine batches of Atractylodes macrocephala were analyzed to select the chromatographic peaks with good stability,strong absorption,and marked features as common peaks. The results identified seven common peaks,and the similarity of the common peaks was > 0. 96 between the nine batches. Conclusion: Atractylodes macrocephala from the three producing areas of Zhejiang,Hunan,Anhui meets the quality standard and has no difference in fingerprints,suggesting that there is no significant difference in their quality,and identification and differentiation cannot be performed.